Overview

  • Product nameAnti-NCAM antibody
    See all NCAM primary antibodies
  • Description
    Rabbit polyclonal to NCAM
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse NCAM.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Mouse Hippocampus; Rat Brain; Rat Cerebellum; Rat Forebrain.

Properties

Applications

Our Abpromise guarantee covers the use of ab95153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140,180 kDa (predicted molecular weight: 119 kDa).

Target

  • FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similaritiesContains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody images

  • All lanes : Anti-NCAM antibody (ab95153) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Cerebellum Rat Tissue Lysate
    Lane 6 : Rat Forebrain

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 119 kDa
    Observed band size : 140,180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • IHC image of NCAM staining in Mouse foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95153, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-NCAM antibody (ab95153)

ab95153 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"