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- Secondary antibodies
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Product name
Anti-NCAPG2 antibody
See all NCAPG2 products (3) ...
Description
Rabbit polyclonal to NCAPG2
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between C terminal residues 1100 -1143 of human NCAPG2. (AAH43404.1)
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Western blot - NCAPG2 antibody (ab70350)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: Use at 1-4 µg/mg lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
NCAPG2 is 1 of 3 non-SMC subunits that define condensin II. Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II.
Cellular localization
nucleus
Alternative names
- CAP G2 antibody
- Leucine zipper protein 5 antibody
- Non SMC condensin II complex subunit G2 antibody
see all
Database links
- Entrez Gene: 54892 Human
- Omim: 608532 Human
- SwissProt: Q86XI2 Human
Anti-NCAPG2 antibody images:
Western blot - NCAPG2 antibody (ab70350)
All lanes : Anti-NCAPG2 antibody (ab70350) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 131 kDa
Observed band size : 131 kDa
References for Anti-NCAPG2 antibody (ab70350)
This product has been referenced in:
- Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB; Human.Read more (PubMed: 20622854) »
- Centore RC et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB; Human.Read more (PubMed: 20932472) »
See all 2 publications for this product
Publishing research using ab70350? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"