Anti-NCAPH2 antibody (ab83848)

Overview

  • Product nameAnti-NCAPH2 antibodySee all NCAPH2 primary antibodies ...
  • Description
    Rabbit polyclonal to NCAPH2
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (EYLYSLVYQA LDFISGKRRA KQLSSVQEDR ANGVASSGVP QEAENEFLSL) of human NCAPH2 (NP_689512).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • FunctionRegulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
  • Sequence similaritiesBelongs to the CND2 H2 (condensin-2 subunit 2) family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP H2 antibody
    • CAP H2 subunit of the condensin II complex antibody
    • CAPH2 antibody
    • Chromosome associated protein H2 antibody
    • Chromosome-associated protein H2 antibody
    • CNDH2_HUMAN antibody
    • Condensin-2 complex subunit H2 antibody
    • hCAP H2 antibody
    • hCAP-H2 antibody
    • Kleisin beta antibody
    • Kleisin-beta antibody
    • Ncaph2 antibody
    • Non SMC condensin II complex subunit H2 antibody
    • Non-SMC condensin II complex subunit H2 antibody
    see all

Anti-NCAPH2 antibody images

  • Anti-NCAPH2 antibody (ab83848) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NCAPH2 antibody (ab83848)

ab83848 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 50 µg
Specification brain
Gel Running Conditions Reduced Denaturing (7)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 20 2013

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