Overview
- Product nameAnti-NCAPH2 antibodySee all NCAPH2 primary antibodies ...
- DescriptionRabbit polyclonal to NCAPH2
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog - Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (EYLYSLVYQA LDFISGKRRA KQLSSVQEDR ANGVASSGVP QEAENEFLSL) of human NCAPH2 (NP_689512).
- Positive controlHeLa cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab83848 is purified by a peptide affinity chromatography method.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab83848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ELISA |
ELISA titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionRegulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
- Sequence similaritiesBelongs to the CND2 H2 (condensin-2 subunit 2) family.
- Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Cellular localizationNucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.
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Database links
- Entrez Gene: 767978 Cow
- Entrez Gene: 29781 Human
- Entrez Gene: 52683 Mouse
- Entrez Gene: 300149 Rat
- Omim: 611230 Human
- SwissProt: Q3SZL8 Cow
- SwissProt: Q6IBW4 Human
- SwissProt: Q8BSP2 Mouse
- SwissProt: Q4V8I2 Rat
- Unigene: 180903 Human
- Unigene: 143167 Mouse
- Unigene: 4227 Rat
see all
Target information above from: UniProt accession
Q6IBW4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- CAP H2 antibodyCAP H2 subunit of the condensin II complex antibodyCAPH2 antibody
- Chromosome associated protein H2 antibodyChromosome-associated protein H2 antibodyCNDH2_HUMAN antibodyCondensin-2 complex subunit H2 antibodyhCAP H2 antibodyhCAP-H2 antibodyKleisin beta antibodyKleisin-beta antibodyNcaph2 antibodyNon SMC condensin II complex subunit H2 antibodyNon-SMC condensin II complex subunit H2 antibody
see all
Anti-NCAPH2 antibody images
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Western blot - NCAPH2 antibody (ab83848)Anti-NCAPH2 antibody (ab83848) at 1 µg/ml + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.
References for Anti-NCAPH2 antibody (ab83848)
ab83848 has not yet been referenced specifically in any publications.
