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Overview

  • Product nameNCAPH2 protein (Tagged-His Tag)See all NCAPH2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 205-605 (401 amino acids) of Human NCAPH2 with an N-terminal His tag; Predicted MWt 46 kDa (UniProt Q6IBW4).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 78 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesAGVSPMPGTQKDTGRTEEQPMEVSVCRSPVPALGFSQEPG PSPEGPMPL GGGEDEDAEEAVELPEASAPKAALEPKES RSPQQSAALPRRYMLREREG APEPASCVKETPDPWQSL DPFDSLESKPFKKGRPYSVPPCVEEALGQKR KRKGAAK LQDFHQWYLAAYADHADSRRLRRKGPSFADMEVLYWTHVK EQ LETLRKLQRREVAEQWLRPAEEDHLEDSLEDLGAAD DFLEPEEYMEPEG ADPREAADLDAVPMSLSYEELVRRN VELFIATSQKFVQETELSQRIRDW EDTVQPLLQEQEQH VPFDIHTYGDQLVSRFPQLNEWCPFAELVAGQPAF EVC RSMLASLQLANDYTVEITQQPGLEMAVDTMSLRLLTHQRA HKRFQT YAAPSMAQP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91812 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CAP H2CAP H2 subunit of the condensin II complexCAPH2
        Chromosome associated protein H2Chromosome-associated protein H2CNDH2_HUMANCondensin-2 complex subunit H2hCAP H2hCAP-H2Kleisin betaKleisin-betaNcaph2Non SMC condensin II complex subunit H2Non-SMC condensin II complex subunit H2
      see all
  • FunctionRegulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
  • Sequence similaritiesBelongs to the CND2 H2 (condensin-2 subunit 2) family.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.

    • Target information above from: UniProt accession Q6IBW4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    NCAPH2 protein (Tagged-His Tag) images

    • SDS-PAGE - NCAPH2 protein (Tagged-His Tag) (ab91812)
      SDS-PAGE - NCAPH2 protein (Tagged-His Tag) (ab91812)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for NCAPH2 protein (Tagged-His Tag) (ab91812)

    ab91812 has not yet been referenced specifically in any publications.

    Publishing research using ab91812 ? Please let us know so that we can cite the reference in this datasheet

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