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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-NCBP1 antibody
See all NCBP1 products (4) ...
Description
Rabbit polyclonal to NCBP1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Xenopus laevis, Cynomolgus Monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NCBP1.
(Peptide available as ab42388.)
Positive control
ab42389 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HepG2 (Human hepatocellular liver carcinoma cell line) A431 (Human epithelial carcinoma cell line) ab42389 gave a positive result in the following nuclear lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HepG2 (Human hepatocellular liver carcinoma cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.
Sequence similarities
Belongs to the NCBP1 family.
Contains 1 MIF4G domain.
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accessionQ09161
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- nuclear cap binding protein subunit 1 antibody
- 80 kDa nuclear cap-binding protein antibody
- CBP80 antibody
see all
Database links
- Entrez Gene: 427379 Chicken
- Entrez Gene: 4686 Human
- Entrez Gene: 433702 Mouse
- Entrez Gene: 298075 Rat
- Omim: 600469 Human
- SwissProt: Q09161 Human
- SwissProt: Q5T7W5 Human
- SwissProt: Q5T7X2 Human
see all
Anti-NCBP1 antibody images:
Western blot - NCBP1 antibody (ab42389)
All lanes : Anti-NCBP1 antibody (ab42389) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 92 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
ab42389 detects a band at 80 kDa which corresponds to the NCBP 80 kDa subunit.
Immunocytochemistry/ Immunofluorescence - NCBP1 antibody (ab42389)
ICC/IF image of ab42389 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42389, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-NCBP1 antibody (ab42389)
This product has been referenced in:
- Haedicke Jet al. The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection. Proc Natl Acad Sci U S A 106:14040-5 (2009). WB; Human.Read more (PubMed: 19667186) »
See 1 publication for this product
Publishing research using ab42389? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"