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Western blot - NCBP1 antibody (ab64837)
(enlarge)
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Product Name
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NCBP1 antibody
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See all NCBP1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NCBP1
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Immunogen
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Synthetic peptide derived from N-terminal of human NCBP1.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab64837 detects endogenous levels of total NCBP1 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HT29 and LOVO cell extracts.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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NCBP1 (also known as CBP80) is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NCBP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NCBP1 antibody images, references, products related to ab64837 and other tools.
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NCBP1 antibody images:
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 Western blot - NCBP1 antibody (ab64837)
All lanes : NCBP1 antibody (ab64837) at 1/500 dilution
Lane 1 : HT29 cell extract
Lane 2 : LOVO cell extract
Lane 3 : LOVO cell extract with immunizing peptide
Predicted band size : 80 kDa
Observed band size : 80 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
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Search PubMed (MEDLINE) for references to NCBP1
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NCBP1 antibody - more information
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Customer reviews (feedback) regarding NCBP1 antibody |
Customer FAQs regarding NCBP1 antibody |
Protocols for NCBP1 antibody |
Price and availability of products related to NCBP1 antibody |
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NCBP1 antibody - related products:
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NCBP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NCBP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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