![Western blot - NCBP2 antibody [2613C3a] (ab84854) image](#NCBP2-Primary-antibodies-ab84854-1.jpg)
![Immunocytochemistry/ Immunofluorescence - NCBP2 antibody [2613C3a] (ab84854) image](#NCBP2-Primary-antibodies-ab84854-2.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-NCBP2 antibody [2613C3a]
See all NCBP2 products (6) ...
Description
Mouse monoclonal [2613C3a] to NCBP2
Tested applications
Dot Blot, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: RGDNEEQEKL LKKSCTLYVG NLSFYTTEEQ IYELFSKSGD IKKIIMGLDK MKKTACGFCF VEYYSRADAE NAMRYINGTR LDDRIIRTDW DAGFKEGRQY GRGRSGGQV, corresponding to internal sequence amino acids 26-134 of Human NCBP2
RGDNEEQEKL LKKSCTLYVG NLSFYTTEEQ IYELFSKSGD IKKIIMGLDK MKKTACGFCF VEYYSRADAE NAMRYINGTR LDDRIIRTDW DAGFKEGRQY GRGRSGGQV
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab84854 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Clonality
Monoclonal
Clone number
2613C3a
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
Target
Relevance
NCBP2 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. It has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- 20 kDa nuclear cap binding protein antibody
- CBC2 antibody
- CBP20 antibody
see all
Database links
- Entrez Gene: 22916 Human
- Omim: 605133 Human
- SwissProt: P52298 Human
- Unigene: 591671 Human
Anti-NCBP2 antibody [2613C3a] images:
Western blot - NCBP2 antibody [2613C3a] (ab84854)
![Western blot - NCBP2 antibody [2613C3a] (ab84854)](/ps/datasheet/images/84/ab84854/NCBP2-Primary-antibodies-ab84854-1.jpg)
Anti-NCBP2 antibody [2613C3a] (ab84854) + Immunogen (Recombinant Human NCBP2 fragment)
Predicted band size : 18 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NCBP2 antibody [2613C3a] (ab84854)
![Immunocytochemistry/ Immunofluorescence - NCBP2 antibody [2613C3a] (ab84854)](/ps/datasheet/images/84/ab84854/NCBP2-Primary-antibodies-ab84854-2.jpg)
ab84854 staining NCBP2 in HeLa cells by Immunofluorescence. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS.
References for Anti-NCBP2 antibody [2613C3a] (ab84854)
ab84854 has not yet been referenced specifically in any publications.
Publishing research using ab84854? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"