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- Proteins and Peptides
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Product name
Anti-NCE2 antibody
See all NCE2 products (7) ...
Description
Goat polyclonal to NCE2
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human, Xenopus laevis
Immunogen
Synthetic peptide: RNKVDDYIKRYAR, corresponding to C terminal amino acids 173-185 of Human NCE2.
C-RNKVDDYIKRYAR
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - NCE2 antibody (ab15707)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/500 (PMID 19250909).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue specificity
Widely expressed (at protein level).
Pathway
Protein modification; protein neddylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
Target information above from: UniProt accessionQ969M7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hypothetical protein NCE2 antibody
- MGC18120 antibody
- NCE2 antibody
see all
Database links
- Entrez Gene: 140739 Human
- Entrez Gene: 67921 Mouse
- Entrez Gene: 432085 Xenopus laevis
- SwissProt: Q969M7 Human
- SwissProt: Q9CY34 Mouse
- SwissProt: Q6IRC7 Xenopus laevis
- Unigene: 471785 Human
- Unigene: 337238 Mouse
see all
Anti-NCE2 antibody images:
Western blot - NCE2 antibody (ab15707)
All lanes : Anti-NCE2 antibody (ab15707) at 1/1000 dilution
Lane 1 : Endogenous Nce2 from mouse 3T3 cells
Lane 2 : NIH 3T3 cells infected with ShRNA of mouse Nce2
Lane 3 : NIH3T3 cells infected with ShRNA of Nce2 and MCSV-NCE2his flag-IRES-GFP (human NCE2)
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated rabbit polyclonal to goat IgG
developed using the ECL technique
Exposure time : 10 minutes
The image is a courtsey of an abreview submitted by Dr. Martine Roussel.
References for Anti-NCE2 antibody (ab15707)
This product has been referenced in:
- Huang DT et al. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell 33:483-95 (2009). WB.Read more (PubMed: 19250909) »
See 1 publication for this product
Publishing research using ab15707? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"