You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-NCKIPSD antibody [2614C1a] (ab58676)

Submit an Abreview Q&A (2)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab58676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 79 kDa.
Dot Blot Dot: Use at an assay dependent dilution.

Target

  • FunctionHas an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.
  • Tissue specificityHighest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
  • Involvement in diseaseNote=A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL.
  • Sequence similaritiesContains 1 SH3 domain.
  • Cellular localizationNucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.

    • Target information above from: UniProt accession Q9NZQ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        54 kDa VacA-interacting protein antibody54 kDa vimentin-interacting protein antibody90 kDa SH3 protein interacting with Nck antibody
        AF3p21 antibodydia interacting protein antibodyDia-interacting protein 1 antibodyDiaphanous protein-interacting protein antibodyDIP antibodyDIP-1 antibodyDIP1 antibodyNCK interacting protein with SH3 domain antibodyNCK-interacting protein with SH3 domain antibodyNckipsd antibodyORF1 antibodySH3 adapter protein SPIN90 antibodySH3 protein interacting with Nck 90 kDa antibodySPIN90 antibodySPN90_HUMAN antibodyVIP54 antibodyWASLBP antibodyWASP-interacting SH3-domain protein antibodyWISH antibodywiskott- Aldrich syndrome protein-interacting protein antibodyWiskott-Aldrich syndrome protein-interacting protein antibody
      see all

    Anti-NCKIPSD antibody [2614C1a] images

    References for Anti-NCKIPSD antibody [2614C1a] (ab58676)

    ab58676 has not yet been referenced specifically in any publications.

    Publishing research using ab58676 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab58676 Submit a Question for ab58676

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    I would love to try another vial, in an new lot if possible. If not, I can test the lot again to see if the problem was specific to the vial. Thanks

    Thank you for reply, and I apologize again for the variability in the results with this antibody. I am sending a free of charge replacement vial on the order ***. We currently are out of stock of ab58676, so the expected delivery date is December 14. All ...

    Read More

    DESCRIPTION OF THE PROBLEM No signal or weak signal upon overexpression of full-length protein no bands detected with endogenous proteins SAMPLE HEK293T whole cell lysate of overexpresses HA-tagged full length DIP protein 30ug PRIMARY ANTIBODY ab58676 1:5...

    Read More

    Thank you for contacting us and letting us know about the problem with this antibody. I am sorry to hear that this new lot is not working as expected. We do guarantee all our products to worked in tested species and applications for up to 6 months after p...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"