NCoR2 antibody [1212] (ab2780)

Product Name 

NCoR2 antibody [1212]

Product type 

Primary antibodies

Description 

Mouse monoclonal [1212] to NCoR2

Immunogen 

Synthetic peptide: CGGA-SASDPHREKTQ, corresponding to amino acids 994-1005 of Human NCoR 2.
BLAST 'CGGA-SASDPHREKTQ' with or

Reacts with 

Hu

Does not react with 
(species key)

Rat

Tested applications 
(see key)

IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/50.
WB: 1/250. Detects a band of approximately 220-250 kDa (predicted molecular weight: 274 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors

Relevance 

NCOR2 (Nuclear receptor corepressor 2) is a corepressor that mediates transcriptional repression activity of some nuclear receptors. NCOR2 is a component of a large corepressor complex that contains SIN3A/B and the histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and retinoic acid receptors (TR and RAR, respectively) in the absence of their ligands. NCOR2 contains two conserved CORNR box motifs, which are essential and sufficient to permit binding to unliganded TR and RAR. Sequences that surround the CORNR box determine specificity for the nuclear receptor. The interaction between NCOR2 and unliganded nuclear receptor is thought to promote the association of these nuclear receptors with TFIIB. NCOR2 is also a component of the N-Cor repressor complex, which also contains NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A, and GPS2.

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing Mediator of Retinoic acid and Thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1212

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS, 1 mg/ml BSA, 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for NCoR2 antibody [1212] images, references, products related to ab2780 and other tools.

NCoR2 antibody [1212] images:

References for NCoR2 antibody [1212] (ab2780)

  This product has been used in: (two most recent references)

Yu J et al. The corepressor silencing mediator for retinoid and thyroid hormone receptor facilitates cellular recovery from DNA double-strand breaks. Cancer Res 66:9316-22 (2006). WB. PubMed: 16982777

Mani S et al. Activation of the steroid and xenobiotic receptor (human pregnane X receptor) by nontaxane microtubule-stabilizing agents. Clin Cancer Res 11:6359-69 (2005). PubMed: 16144941
If you publish research using ab2780 please let us know so that we can cite the reference on this datasheet.

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