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Overview
Product name
Anti-NCOR2 antibody [1542/H7]
See all NCOR2 products (7) ...
Description
Mouse monoclonal [1542/H7] to NCOR2
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: ASADPHREKTQ conjugated to KLH, corresponding to amino acids 994-1005 of Human NCoR2.
ASADPHREKT Q
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibody purified from hybridoma cell culture supernatant by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE. Prepared in 10mM PBS (pH 7.4).
Primary antibody notes
The Silencing Mediator for Retinoid and Thyriod receptors (SMRT) gene product is a 168 kDa nuclear protein. Nuclear hormone receptors are transcription factors regulated by ligand binding. Use at 1-5 µg/ml. Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing Mediator of Retinoic acid and Thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
Clonality
Monoclonal
Clone number
1542/H7
Myeloma
unknown
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Application notes
IP: Use at an assay dependant concentration.
WB: Use at an assay dependant dilution. Predicted molecular weight: 274 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
Tissue specificity
Ubiquitous. High levels of expression are detected in lung, spleen and brain.
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.
Domain
The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTG repeat protein 26 antibody
- retinoic-acid-receptor-associated corepressor antibody
- SMAP270 antibody
see all
Database links
- Entrez Gene: 9612 Human
- Omim: 600848 Human
- SwissProt: Q9Y618 Human
- Unigene: 137510 Human
References for Anti-NCOR2 antibody [1542/H7] (ab85)
This product has been referenced in:
- Racanicchi Set al. Targeting fusion protein/corepressor contact restores differentiation response in leukemia cells. EMBO J 24:1232-42 (2005).Read more (PubMed: 15729358) »
See 1 publication for this product
Publishing research using ab85? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"