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Overview
Product name
Anti-NCOR2 antibody - ChIP Grade
See all NCOR2 products (7) ...
Description
Rabbit polyclonal to NCOR2 - ChIP Grade
Specificity
Does not react with NCoR.
Tested applications
ChIP, ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 1165-1363 of Human NCoR2
Positive control
HeLa nuclear extract
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
ICC/IF: 1/300
IP: Use at an assay dependent dilution.
WB: 1/1000Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).
Target
Function
Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
Tissue specificity
Ubiquitous. High levels of expression are detected in lung, spleen and brain.
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.
Domain
The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTG repeat protein 26 antibody
- retinoic-acid-receptor-associated corepressor antibody
- SMAP270 antibody
see all
Database links
- Entrez Gene: 9612 Human
- Omim: 600848 Human
- SwissProt: Q9Y618 Human
- Unigene: 137510 Human
Anti-NCOR2 antibody - ChIP Grade images:
Western blot - NCoR2 antibody - ChIP Grade (ab24551)
Anti-NCOR2 antibody - ChIP Grade (ab24551) at 1/1000 dilution + HeLa nuclear extract
Predicted band size : 274 kDa
Observed band size : 270 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NCOR2 antibody - ChIP Grade (ab24551)
ab24551 staining NCOR2 (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-NCOR2 antibody - ChIP Grade (ab24551)
This product has been referenced in:
- Stockert Jet al. Reverse crosstalk of TGF{beta} and PPAR{beta}/{delta} signaling identified by transcriptional profiling. Nucleic Acids Res 39:119-31 (2011). ChIP; Human.Read more (PubMed: 20846954) »
- Battaglia Set al. Elevated NCOR1 disrupts PPARalpha/gamma signaling in prostate cancer and forms a targetable epigenetic lesion. Carcinogenesis 31:1650-60 (2010). WB; Human.Read more (PubMed: 20466759) »
See all 6 publications for this product
Publishing research using ab24551? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"