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Overview
Product name
Anti-NCoR2 antibody
See all NCOR2 products (7) ...
Description
Rabbit polyclonal to NCoR2
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: PAPPAPPPPQNL, corresponding to amino acids 994-1005 of Human NCoR2
PAPPAPPPPQ NL
Positive control
Human brain tissue and human lung carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NCoR2 antibody (ab74518)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: 1/25 for 10 minutes at room temperature.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
Tissue specificity
Ubiquitous. High levels of expression are detected in lung, spleen and brain.
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.
Domain
The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y618
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTG repeat protein 26 antibody
- retinoic-acid-receptor-associated corepressor antibody
- SMAP270 antibody
see all
Database links
- Entrez Gene: 9612 Human
- Omim: 600848 Human
- SwissProt: Q9Y618 Human
- Unigene: 137510 Human
Anti-NCoR2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NCoR2 antibody (ab74518)
ab74518, at 1/25 dilution, staining NCoR2 in formalin fixed, paraffin embedded human brain tissue by Immunohistochemistry.
References for Anti-NCoR2 antibody (ab74518)
ab74518 has not yet been referenced specifically in any publications.
Publishing research using ab74518? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"