Overview
Product nameNDE1 protein (His tag)See all NDE1 proteins and peptides ...
Protein descriptionRecombinant full length Human NDE1 (amino acids 1-335) with N terminal His tag; 355 amino acids with tag, single non-glycosylated polypeptide chain, MWt 39.9 kDa (UniProt Q9NXR1-2).
Uniprot accessionQ9NXR1-2
Molecular weight39.900kDa inclusive of tags
Protein length335 amino acids
Expression hostE. coli
Properties
Purity> 85
% by SDS-PAGE
Purification notesab125486 is purified by proprietary chromatographic techniques. Purity is greater than 85.0% as determined by SDS-PAGE.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.08% DTT, 0.32% Tris HCl, 20% Glycerol, 1.17% Sodium chloride
Concentration information loading...
Sequence notesMGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab125486
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
FLJ20101HOM TES 87LIS1 interacting protein NUDE1
LIS1 interacting protein NUDE1 rat homologLIS4NDE 1NDE1NDE1_HUMANNuclear distribution gene E homolog 1Nuclear distribution protein nudE homolog 1NudENUDE 1NudE nuclear distribution gene E homolog 1NudE nuclear distribution gene E homolog 1 (A. nidulans)NUDE1
see all
FunctionRequired for centrosome duplication. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex (By similarity). Required for formation and function of the mitotic spindle.
Sequence similaritiesBelongs to the nudE family.
Post-translational
modificationsPhosphorylated in mitosis.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Localizes to the interphase centrosome and to the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis.
References for NDE1 protein (His tag) (ab125486)
ab125486
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
