Recombinant Anti-NDUFA10 antibody [EPR12135] (ab174829)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12135] to NDUFA10
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NDUFA10 antibody [EPR12135]
See all NDUFA10 primary antibodies -
Description
Rabbit monoclonal [EPR12135] to NDUFA10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa, Jurkat, Raji and Human fetal heart lysates; Human heart and kidney tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12135 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa). |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. -
Cellular localization
Mitochondrion matrix. -
Database links
- Entrez Gene: 4705 Human
- Omim: 603835 Human
- SwissProt: O95299 Human
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Alternative names
- CI 42KD antibody
- CI-42k antibody
- Complex I-42kD antibody
see all
Images
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All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).
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Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab174829 has been referenced in 3 publications.
- Wang Z et al. High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma. Transl Oncol 14:101146 (2021). PubMed: 34118692
- Basit F et al. Human Dendritic Cell Subsets Undergo Distinct Metabolic Reprogramming for Immune Response. Front Immunol 9:2489 (2018). PubMed: 30455688
- Khorsandi SE et al. Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers. BMC Res Notes 10:547 (2017). PubMed: 29096719