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Overview

  • Product nameNDUFA10 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 171-355 (185) of Human NDUFA10 with N terminal His tag; MWt 24kDa; UniProt ID = O95299
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His- tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 65 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS)(acquired on initial reference batch)
  • Sequence notesEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVP VPEVQRRIQKKGDP HEMKITSAYLQDIENAYKKTFLPE MSEKCEVLQYSAREAQDSKKVVEDIEYLKF DKGPWLKQ DNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDR VLHQFR ELPGRKYSPGYNTEVGDKWIWLK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91702 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CI 42KDCI-42kComplex I-42kD
        MGC5103NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDaNADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrialNADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursorNADH-ubiquinone oxidoreductase 42 kDa subunitndufa10l
      see all
  • RelevanceNDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
  • Cellular localizationMitochondrion matrix.

    • NDUFA10 protein (Tagged-His Tag) images

    • SDS-PAGE - NDUFA10 protein (Tagged-His Tag) (ab91702)
      SDS-PAGE - NDUFA10 protein (Tagged-His Tag) (ab91702)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for NDUFA10 protein (Tagged-His Tag) (ab91702)

    ab91702 has not yet been referenced specifically in any publications.

    Publishing research using ab91702 ? Please let us know so that we can cite the reference in this datasheet

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