Overview
- Product nameAnti-NDUFB10 antibodySee all NDUFB10 primary antibodies ...
- DescriptionRabbit polyclonal to NDUFB10
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide derived from an internal sequence of human NDUFB10
- Positive controlExtracts from COLO 205 cells, Jurkat cells, HeLa cells, HepG2 cells or HUVEC cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab74940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
| ELISA | ELISA: 1/40000. |
Target
- FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
- Sequence similaritiesBelongs to the complex I NDUFB10 subunit family.
- Cellular localizationMitochondrion inner membrane.
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Database links
- Entrez Gene: 4716 Human
- Omim: 603843 Human
- SwissProt: O96000 Human
- Unigene: 513266 Human
Target information above from: UniProt accession
O96000
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 0610011B04Rik antibody22kDa antibodyCI PDSW antibody
- CI-PDSW antibodyComplex I PDSW antibodyComplex I-PDSW antibodyNADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 antibodyNADH ubiquinone oxidoreductase PDSW subunit antibodyNADH-ubiquinone oxidoreductase PDSW subunit antibodyNDUBA_HUMAN antibodyNDUFB 10 antibodyNdufb10 antibodyPDSW antibody
see all
Anti-NDUFB10 antibody images
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All lanes : Anti-NDUFB10 antibody (ab74940) at 1/500 dilution
Lane 1 : Extracts from COLO 205 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from HeLa cells
Lane 4 : Extracts from HepG2 cells
Lane 5 : Extracts from HUVEC cells
Lane 6 : Extracts from COLO 205 cells with immunizing peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size : 21 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
References for Anti-NDUFB10 antibody (ab74940)
ab74940 has not yet been referenced specifically in any publications.
