Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-NDUFB4 antibody
See all NDUFB4 products (3) ...
Description
Mouse monoclonal to NDUFB4
Tested applications
WB, ICC/IF, Flow Cytmore details
Cross reactivity
Reacts with
Rat, Cow, Human
Immunogen
NDUFB4 from cow
Positive control
Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF: Use a concentration of 5 - 15 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal).
Target
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similarities
Belongs to the complex I NDUFB4 subunit family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionO95168
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B15 antibody
- CI-B15 antibody
- Complex I-B15 antibody
see all
Database links
- Entrez Gene: 4710 Human
- Entrez Gene: 288088 Rat
- Omim: 603840 Human
- SwissProt: O95168 Human
- Unigene: 304613 Human
Anti-NDUFB4 antibody images:
Western blot - Anti-NDUFB4 antibody (ab110243)
All lanes : Anti-NDUFB4 antibody (ab110243) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Predicted band size : 15 kDa
Immunocytochemistry/ Immunofluorescence - NDUFB4 antibody (ab110243)
Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG.
Flow Cytometry - NDUFB4 antibody (ab110243)
HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
References for Anti-NDUFB4 antibody (ab110243)
This product has been referenced in:
- Ferreira M et al. Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1. Neurogenetics 12:9-17 (2011).Read more (PubMed: 21203893) »
- Thomas RR et al. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18 (2011).Read more (PubMed: 20727424) »
See all 8 publications for this product
Publishing research using ab110243? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"