Anti-NDUFB4 antibody [17G3D9E12] (ab110243)

Overview

• Product nameAnti-NDUFB4 antibody [17G3D9E12]See all NDUFB4 primary antibodies ...
• Description
  Mouse monoclonal [17G3D9E12] to NDUFB4
• Tested applicationsIHC-P, WB, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Rat, Cow, Human
  
• Immunogen

  NDUFB4 from cow

• Positive controlIsolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
• Concentration information loading...
• Purity>95% by SDS-PAGE
• Purification notesNear homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
• Clonality Monoclonal
• Clone number17G3D9E12
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex I

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
• Sequence similaritiesBelongs to the complex I NDUFB4 subunit family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession O95168 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4710 Human
  • Entrez Gene: 288088 Rat
  • Omim: 603840 Human
  • SwissProt: O95168 Human
  • Unigene: 304613 Human

• Alternative names
  B15 antibodyCI-B15 antibodyComplex I-B15 antibody

  NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa antibodyNADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibodyNADH ubiquinone oxidoreductase B15 subunit antibodyNADH-ubiquinone oxidoreductase B15 subunit antibodyNDUB4_HUMAN antibodyNDUFB4 antibody

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Anti-NDUFB4 antibody [17G3D9E12] images

• 

  Western blot - Anti-NDUFB4 antibody (ab110243)
  All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml
  
  Lane 1 : Isolated mitochondria from Human heart at 5 µg
  Lane 2 : Isolated mitochondria from cow heart at 1 µg
  Lane 3 : Isolated mitochondria from rat heart at 10 µg
  
  
  Predicted band size : 15 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - NDUFB4 antibody (ab110243)
  Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
  Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG.
• 

  Flow Cytometry - NDUFB4 antibody (ab110243)
  HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)Philippa Hepplewhite, University of Newcastle, UK
  Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.