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Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

MSCatalog No. MS105

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Overview

Product name

Anti-NDUFB8 antibody [20E9DH10C12]
See all NDUFB8 products (3) ...

Description

Mouse monoclonal [20E9DH10C12] to NDUFB8

Tested applications

WB, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

NDUFB8 from Cow

Positive control

Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HEPES

Concentration

Concentration information loading...

Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

20E9DH10C12

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial

  • Western blot - NDUFB8 antibody [20E9DH10C12] (ab110242)Western blot - NDUFB8 antibody [20E9DH10C12] (ab110242) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Sequence similarities

Belongs to the complex I NDUFB8 subunit family.

Cellular localization

Mitochondrion inner membrane.

Target information above from: UniProt accessionO95169 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • ASHI antibody
  • CI-ASHI antibody
  • Complex I ASHI subunit antibody
  • Complex I-ASHI antibody
  • MS105 antibody
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
  • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
  • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
  • NADH-ubiquinone oxidoreductase ASHI subunit antibody
  • NDUB8_HUMAN antibody
  • NDUFB8 antibody
see all

Database links

Anti-NDUFB8 antibody [20E9DH10C12] images:

  Western blot - NDUFB8 antibody [20E9DH10C12] (ab110242)

Western blot - NDUFB8 antibody [20E9DH10C12] (ab110242)

All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from mouse heart at 10 µg


Predicted band size : 22 kDa


Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

  Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.

References for Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

This product has been referenced in:

  • Thomas RR  et al. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18 (2011).Read more (PubMed: 20727424) »
  • Ferreira M  et al. Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1. Neurogenetics 12:9-17 (2011).Read more (PubMed: 21203893) »

See all 26 publications for this product

Publishing research using ab110242? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"