![Western blot - NDUFS3 antibody [17D95] (ab14711) image](#NDUFS3-Primary-antibodies-ab14711-2.jpg)
 image](#NDUFS3-Primary-antibodies-ab14711-3.jpg)
![Western blot - NDUFS3 antibody [17D95] (ab14711) image](#NDUFS3-Primary-antibodies-ab14711-5.jpg)
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Read our guarantee »Overview
Product name
Anti-NDUFS3 antibody [17D95]
See all NDUFS3 products (4) ...
Description
Mouse monoclonal [17D95] to NDUFS3
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Zebrafish
Immunogen
Purified cow mitochondrial complex I.
Positive control
Human heart mitochondria.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: HEPES buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
17D95
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similarities
Belongs to the complex I 30 kDa subunit family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionO75489
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- CI 30KD antibody
- CI-30kD antibody
see all
Database links
- Entrez Gene: 287327 Cow
- Entrez Gene: 4722 Human
- Entrez Gene: 68349 Mouse
- Entrez Gene: 295923 Rat
- Entrez Gene: 550451 Zebrafish
- Omim: 603846 Human
- SwissProt: P23709 Cow
- SwissProt: O75489 Human
see all
Anti-NDUFS3 antibody [17D95] images:
Western blot - NDUFS3 antibody [17D95] (ab14711)
![Western blot - NDUFS3 antibody [17D95] (ab14711)](/ps/datasheet/images/14/ab14711/NDUFS3-Primary-antibodies-ab14711-2.jpg)
All lanes : Anti-NDUFS3 antibody [17D95] (ab14711) at 1 µg/ml
Lane 1 :
Lane 2 :
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
The band observed at 26 kDa could potentially be a cleaved form of NDUFS3 due to the presence of a 36 amino acid transit peptide.
Flow Cytometry-NDUFS3 antibody [17D95](ab14711)
](/ps/datasheet/images/14/ab14711/NDUFS3-Primary-antibodies-ab14711-3.jpg)
Overlay histogram showing HepG2 cells stained with ab14711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14711, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - NDUFS3 antibody [17D95] (ab14711)
![Western blot - NDUFS3 antibody [17D95] (ab14711)](/ps/datasheet/images/14/ab14711/NDUFS3-Primary-antibodies-ab14711-5.jpg)
All lanes : Anti-NDUFS3 antibody [17D95] (ab14711)
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Secondary
Goat anti-Mouse IgG
Predicted band size : 30 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
References for Anti-NDUFS3 antibody [17D95] (ab14711)
This product has been referenced in:
- van den Ecker D et al. Identification and functional analysis of mitochondrial complex I assembly factor homologues in C. elegans. Mitochondrion : (2012).Read more (PubMed: 22387847) »
- Tuppen HA et al. The p.M292T NDUFS2 mutation causes complex I-deficient Leigh syndrome in multiple families. Brain 133:2952-63 (2010).Read more (PubMed: 20819849) »
See all 23 publications for this product
Publishing research using ab14711? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"