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Whole mitochondria from Human Heart.
Our Abpromise guarantee covers the use of ab110246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25223649|
|Flow Cyt||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.|
Isotype control antibody, at 1 µg/ml, staining (red).
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"