The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 25223649
Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 5 - 10 µg/ml.
1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I 30 kDa subunit family.
NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody
Anti-NDUFS3 antibody [3F9DD2] images
Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)This image is courtesy of an anonymous AbReview
All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 1/1000 dilution
Lane 1 : Whole cell lysates from NDUFS3 KO cells. Lane 2 : Whole cell lysates from NDUFS3 KO cells. Lane 3 : Whole cell lysates from NDUFS3 WT cells. Lane 4 : Whole cell lysates from NDUFS3 WT cells. Lane 5 : Isolated mitochondria from NDUFS3 KO cells. Lane 6 : Isolated mitochondria from NDUFS3 KO cells. Lane 7 : Isolated mitochondria from NDUFS3 WT cells. Lane 8 : Isolated mitochondria from NDUFS3 WT cells.
Lysates/proteins at 50 µg per lane.
Secondary Goat anti-Mouse monoclonal HRP conjugate at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa Additional bands at : 260 kDa (possible non-specific binding),40 kDa (possible non-specific binding).
Western blot - NDUFS3 antibody [3F9DD2 ] (ab110246)
All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg Lane 2 : Isolated mitochondria from Bovine heart at 1 µg Lane 3 : Isolated mitochondria from Rat heart at 10 µg Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.
ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.
ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.
ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).
References for Anti-NDUFS3 antibody [3F9DD2] (ab110246)
This product has been referenced in:
Shephard F et al. A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson's disease. Mitochondrion14:64-72 (2014).
Read more (PubMed: 24333691) »
Ng AC et al. Essential role of TID1 in maintaining mitochondrial membrane potential homogeneity and mitochondrial DNA integrity. Mol Cell Biol34:1427-37 (2014).
Read more (PubMed: 24492964) »