Anti-NDUFS3 antibody [3F9DD2] (ab110246)

Overview

• Product nameAnti-NDUFS3 antibody [3F9DD2]See all NDUFS3 primary antibodies ...
• Description
  Mouse monoclonal [3F9DD2] to NDUFS3
• Tested applicationsIHC-Glut, Flow Cyt, ICC/IF, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human, Zebrafish
  
• Immunogen

  Whole mitochondria from Human Heart.

• Positive controlIsolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: HBS
• Concentration information loading...
• PurityIgG fraction
• Purification notesab110246 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110246 is judged as near homogeneity by SDS PAGE.
• Clonality Monoclonal
• Clone number3F9DD2
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex I

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
• Sequence similaritiesBelongs to the complex I 30 kDa subunit family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession O75489 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 287327 Cow
  • Entrez Gene: 4722 Human
  • Entrez Gene: 68349 Mouse
  • Entrez Gene: 295923 Rat
  • Entrez Gene: 550451 Zebrafish
  • Omim: 603846 Human
  • SwissProt: P23709 Cow
  • SwissProt: O75489 Human

  • SwissProt: Q9DCT2 Mouse
  • Unigene: 502528 Human
  • Unigene: 30113 Mouse

  see all

• Alternative names
  CI 30 antibodyCI 30KD antibodyCI-30kD antibody

  Complex I 30KD antibodyComplex I 30kDa subunit antibodyCOMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibodyComplex I-30kD antibodymitochondrial antibodyNADH coenzyme Q reductase antibodyNADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibodyNADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibodyNADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibodyNADH dehydrogenase ubiquinone 30 kDa subunit antibodyNADH-ubiquinone oxidoreductase 30 kDa subunit antibodyNADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibodyNDUFS3 antibodyNDUS3_HUMAN antibody

  see all

Anti-NDUFS3 antibody [3F9DD2] images

• 

  Western blot - NDUFS3 antibody [3F9DD2 ] (ab110246)
  All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml
  
  Lane 1 : Isolated mitochondria from Human heart at 5 µg
  Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
  Lane 3 : Isolated mitochondria from Rat heart at 10 µg
  Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
  
  
  Predicted band size : 30 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.
• 

  Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue).

  Isotype control antibody, at 1 µg/ml, staining (red).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.

  ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.

  ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).