![Western blot - NDUFS3 antibody [3F9DD2 ] (ab110246) image](#NDUFS3-Primary-antibodies-ab110246-1.JPG)
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246) image](#NDUFS3-Primary-antibodies-ab110246-9.jpg)
![Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246) image](#NDUFS3-Primary-antibodies-ab110246-10.gif)
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Product name
Anti-NDUFS3 antibody [3F9DD2]
See all NDUFS3 products (4) ...
Description
Mouse monoclonal [3F9DD2] to NDUFS3
Tested applications
IHC-Glut, Flow Cyt, ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Zebrafish
Immunogen
Whole mitochondria from Human Heart.
Positive control
Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
ab110246 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110246 is judged as near homogeneity by SDS PAGE.
Clonality
Monoclonal
Clone number
3F9DD2
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Glut
IHC-Glut: 1/100.IHC-Glut: 1/100.
Flow Cyt: Use a concentration of 1 µg/ml.
ICC/IF: Use a concentration of 5 - 10 µg/ml.
IHC-P: 1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.
Target
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similarities
Belongs to the complex I 30 kDa subunit family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionO75489
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- CI 30KD antibody
- CI-30kD antibody
see all
Database links
- Entrez Gene: 287327 Cow
- Entrez Gene: 4722 Human
- Entrez Gene: 68349 Mouse
- Entrez Gene: 295923 Rat
- Entrez Gene: 550451 Zebrafish
- Omim: 603846 Human
- SwissProt: P23709 Cow
- SwissProt: O75489 Human
see all
Anti-NDUFS3 antibody [3F9DD2] images:
Western blot - NDUFS3 antibody [3F9DD2 ] (ab110246)
![Western blot - NDUFS3 antibody [3F9DD2 ] (ab110246)](/ps/datasheet/images/110/ab110246/NDUFS3-Primary-antibodies-ab110246-1.JPG)
All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Predicted band size : 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246)](/ps/datasheet/images/110/ab110246/NDUFS3-Primary-antibodies-ab110246-9.jpg)
ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.
Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
![Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246)](/ps/datasheet/images/110/ab110246/NDUFS3-Primary-antibodies-ab110246-10.gif)
ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue).
Isotype control antibody, at 1 µg/ml, staining (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)](/ps/datasheet/images/110/ab110246/NDUFS3-Primary-antibodies-ab110246-11.jpg)
ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)](/ps/datasheet/images/110/ab110246/NDUFS3-Primary-antibodies-ab110246-12.jpg)
ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)](/ps/datasheet/images/110/ab110246/NDUFS3-Primary-antibodies-ab110246-13.gif)
ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).
References for Anti-NDUFS3 antibody [3F9DD2] (ab110246)
This product has been referenced in:
- Ferreira M et al. Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1. Neurogenetics 12:9-17 (2011).Read more (PubMed: 21203893) »
- Thomas RR et al. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18 (2011).Read more (PubMed: 20727424) »
See all 25 publications for this product
Publishing research using ab110246? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"