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- Secondary antibodies
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Product name
Anti-NDUFS3 antibody
See all NDUFS3 products (4) ...
Description
Goat polyclonal to NDUFS3
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Cow, Chimpanzee, Gorilla, Orangutan
Immunogen
Peptide with the sequence C-DTRPTVRPRNDVAHK, from the internal region of the Human NDUFS3 (NP_004542.1).
DTRPTVRPRNDVAHK
Positive control
Human Frontal Cortex lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
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Western blot - NDUFS3 antibody (ab87650)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa.
Target
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similarities
Belongs to the complex I 30 kDa subunit family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionO75489
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- CI 30KD antibody
- CI-30kD antibody
see all
Database links
- Entrez Gene: 451175 Chimpanzee
- Entrez Gene: 287327 Cow
- Entrez Gene: 4722 Human
- Omim: 603846 Human
- SwissProt: Q0MQG8 Chimpanzee
- SwissProt: P23709 Cow
- SwissProt: Q0MQG7 Gorilla
- SwissProt: O75489 Human
see all
Anti-NDUFS3 antibody images:
Western blot - NDUFS3 antibody (ab87650)
Anti-NDUFS3 antibody (ab87650) at 0.3 µg/ml + Human Frontal Cortex lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 30 kDa
Observed band size : 30 kDa
Incubate with ab87650 for 1 hour.
References for Anti-NDUFS3 antibody (ab87650)
ab87650 has not yet been referenced specifically in any publications.
Publishing research using ab87650? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"