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NECA Fluorescent ligand (Red) (ab118162)

Q&A (3)

Overview

  • Product nameNECA Fluorescent ligand (Red)
  • Description

    Fluorescent non-selective adenosine agonist

  • Alternative names
    A-633-AGAdenosine-5’-N-ethyluronamide (NECA)-derivative
  • Biological descriptionFluorescent adenosine agonist. Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics. Stimulates phosphoinositide hydrolysis (pEC50 = 7.34). In vitro potencies (pIC50 values) are 8.47 and 8.57 for A1 and A3 respectively. pEC50 values are 6.76 and 5.69 for A2A and A2B respectively.

    Excitation wavelength: 638 nm

    Emission wavelength: 657 nm

  • Purity> 97%

    • Properties

  • PharmacophoreAdenosine-5’-N-ethyluronamide (NECA)-derivative
  • SelectivityA1: 8.47 | A2A: 6.76 | A2B: 5.69 | A3 : 8.57
  • Excitation638nm
  • Emission657nm
  • Molecular Weight925.00
  • Molecular formulaC45H51BF2N10O7S
  • CAS Number774199-07-4
  • Storage instructionsStore at -20°C. Avoid exposure to light.
  • HandlingFor ligand binding, fluorescence imaging and high content analysis, kinetic analysis and cell sorting at A1 / A2A / A3 adenosine receptors use solutions up to 100 nM in DMSO.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab118162 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Small Molecule Fluorescent Optical Detection Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration.
    Receptor Localization Receptor Localization: Use at an assay dependent concentration.
    Fluorescent Cell Imaging Fluorescent Cell Imaging: Use at an assay dependent concentration.

    NECA Fluorescent ligand (Red) images

    • Receptor Localization - NECA Fluorescent ligand (Red) (ab118162)
      Receptor Localization - NECA Fluorescent ligand (Red) (ab118162)
      Left: the ab118162 ligand (30 nM) binding to live CHO cells expressing adenosine A3 receptors. Right: Binding blocked by the unlabelled competitor XAC (10 µM). Nuclei have been counter-stained with Hoechst.
    • Small Molecule Fluorescent Optical Detection - NECA Fluorescent ligand (Red) (ab118162)
      Small Molecule Fluorescent Optical Detection - NECA Fluorescent ligand (Red) (ab118162)
      A1-SPAP cells assayed with ab118162 (A-633-AG)
    • Small Molecule Fluorescent Optical Detection - NECA Fluorescent ligand (Red) (ab118162)
      Small Molecule Fluorescent Optical Detection - NECA Fluorescent ligand (Red) (ab118162)
      A2a-SPAP cells assayed with ab118162 (A-633-AG)
    • Small Molecule Fluorescent Optical Detection - NECA Fluorescent ligand (Red) (ab118162)
      Small Molecule Fluorescent Optical Detection - NECA Fluorescent ligand (Red) (ab118162)
      A3-SPAP cells assayed with ab118162 (A-633-AG)

    References for NECA Fluorescent ligand (Red) (ab118162)

    ab118162 has not yet been referenced specifically in any publications.

    Publishing research using ab118162 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab118162

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    I found you have a panel of fluorescent ligands available at: http://www.abcam.com/index.html?c=5638



    However, I could not find any structure information for them. Is that possible to provide me full structure information for them. I...

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    Thank you for contacting Abcam.

    Unfortunately we are not able to provide the structures of any of these products as this information is proprietary, as the linker is patented.
    These compounds have the benefit that the pharmacological proper...

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    ab118162
    Diluted to 1mM in 54 ul DMSO. Aliquoted and frozen
    Diluted one aliquot in buffer. Buffer on its own and ab118162 gave the same readings on the fluorometer at 638/657 nm
    Suspected buffer interference
    Diluted one aliquot in wate...

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    Thank you for your telephone call.

    I am sorry to hear you have some concerns regarding the NECA Fluorescent ligand (Red). I would like to reassure you that this is covered by our 6 month guarantee.

    I would be pleased to investigate t...

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    I'm very interested, but I need to know the Kd-values of the bodipy ligands listed below:

    Thank you for contacting Abcam.

    I have included a pdf file which contains the pKd values for the products as you have asked. This information and more is also available
    on the individual product pages on the abcam website (e.g. http://www.ab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"