AbcamBiochemicals

NECA Fluorescent ligand (Red) (ab118162)

Overview

  • Product nameNECA Fluorescent ligand (Red)
  • Description

    Fluorescent non-selective adenosine agonist

  • Alternative names
    • A-633-AG
    • Adenosine-5’-N-ethyluronamide (NECA)-derivative
  • Biological descriptionFluorescent adenosine agonist. Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics. Stimulates phosphoinositide hydrolysis (pEC50 = 7.34). In vitro potencies (pIC50 values) are 8.47 and 8.57 for A1 and A3 respectively. pEC50 values are 6.76 and 5.69 for A2A and A2B respectively.

    Excitation wavelength: 638 nm

    Emission wavelength: 657 nm

  • Purity> 97%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab118162 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Small Molecule Fluorescent Optical Detection Use at an assay dependent concentration.
    Receptor Localization Use at an assay dependent concentration.
    Fluorescent Cell Imaging Use at an assay dependent concentration.

    NECA Fluorescent ligand (Red) images

    • Left: the ab118162 ligand (30 nM) binding to live CHO cells expressing adenosine A3 receptors. Right: Binding blocked by the unlabelled competitor XAC (10 µM). Nuclei have been counter-stained with Hoechst.
    • A1-SPAP cells assayed with ab118162 (A-633-AG)
    • A2a-SPAP cells assayed with ab118162 (A-633-AG)
    • A3-SPAP cells assayed with ab118162 (A-633-AG)

    References for NECA Fluorescent ligand (Red) (ab118162)

    ab118162 has not yet been referenced specifically in any publications.

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