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Anti-NEDD4-2 antibody (ab46521)

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1 question for ab46521

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Question 1

Thursday 20-December-2007

We are seeing multiple bands in Western blots with this antibody and are wondering what the bands might represent. Can you provide more details about the immunogen? Is it known if the antibody is reactive with NEDD4?

ANSWER:

  

I have received the following reply from our supplier of this antibody. I hope this information helps. Please contact us if you have any more questions.

The antibody is directed against a region of Nedd4-2 which is specific for Nedd4-2 and does not cross react with Nedd4. The antigen was a recombinant GST-fusion protein containing the following sequence:

LAEDGASGSATNSNNHLVEPQIRRPRSLSSPTVTLSAPLEGAKDSPIRRAVKDTLSNPQSP QPSPYNSPKPQHKVTQ

It was described in: Kamynina, E, Tauxe, C & Staub, O: Differential characteristics of two human Nedd4 proteins with respect to epithelial Na+ channel regulation. Am.J.Physiol., 281:F469-F477, 2001

The appearance of multiple bands are described in several publications:

Chen, H, Ross, CA, Wang, N, Huo, Y, MacKinnon, DF, Potash, JB, Simpson, SG, McMahon, FJ, DePaulo Jr., JR & McInnis, MG: NEDD4L on human chromosome 18q21 has multiple forms of transcripts and is a homologue of the mouse Nedd4-2 gene. Eur.J.Hum.Genet., 9:922-30, 2001

Itani, OA, Campbell, JR, Herrero, J, Snyder, PM & Thomas, CP: Alternative promoter usage and differential splicing leads to human Nedd4-2 isoforms with a C2 domain and with variable number of WW domains. Am.J.Physiol., 285:F916-929, 2003

Dunn, DM, Ishigami, T, Pankow, J, Von Niederhausern, A, Alder, J, Hunt, SC, Leppert, MF, Lalouel, JM & Weiss, RB: Common variant of human NEDD4L activates a cryptic splice site to form a frameshifted transcript. J.Hum.Genet., 47:665-76, 2002

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