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Western blot - NEDD4-2 antibody (ab46521)
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Product Name
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NEDD4-2 antibody
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See all NEDD4-2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NEDD4-2
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Immunogen
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Recombinant fusion protein (Mouse)
LAEDGASGS ATNSNNHLVE PQIRRPRSLS SPTVTLSAPL EGAKDSPIRR AVKDTLSNPQ QPSPYNSPKPQHKVTQ
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Reacts with
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Hu, Ms, Rat
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/200.
WB: 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NEDD4-2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NEDD4-2 antibody images, references, products related to ab46521 and other tools.
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NEDD4-2 antibody images:
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 Western blot - NEDD4-2 antibody (ab46521)
All lanes : NEDD4-2 antibody (ab46521)
Lane 1 : Extract from non-transfected HEK293 cells
Lane 2 : Extract from HEK293 cells transfected with NEDD4-2
Predicted band size : 120 kDa
Observed band size : 120 kDa
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References for NEDD4-2 antibody (ab46521)
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This product has been used in: (two most recent references)
Soundararajan R et al. Epithelial sodium channel regulated by differential composition of a signaling complex. Proc Natl Acad Sci U S A 106:7804-9 (2009). IP; Human. PubMed: 19380724
Caohuy H et al. Rescue of DeltaF508-CFTR by the SGK1/Nedd4-2 signaling pathway. J Biol Chem 284:25241-53 (2009). WB; Human. PubMed: 19617352
See all 4 publication references for this product.
If you publish research using ab46521 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to NEDD4-2
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NEDD4-2 antibody - more information
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Customer reviews (feedback) regarding NEDD4-2 antibody |
Customer FAQs regarding NEDD4-2 antibody |
Protocols for NEDD4-2 antibody |
Price and availability of products related to NEDD4-2 antibody |
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NEDD4-2 antibody - related products:
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NEDD4-2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NEDD4-2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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