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Anti-NEDD4-2 antibody (ab46521)

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Overview

Product name

Anti-NEDD4-2 antibody
See all NEDD4-2 products (6) ...

Description

Rabbit polyclonal to NEDD4-2

Tested applications

ICC, ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, Xenopus laevis

Immunogen

Recombinant fusion protein (Mouse) LAEDGASGSATNSNNHLVEPQIRRPRSLSSP TVTLSAPLEGAKDSPIRRAVKDTLSNPQSP QPSPYNSPKPQHKVTQ

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Western blot - NEDD4-2 antibody (ab46521)Western blot - NEDD4-2 antibody (ab46521) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NEDD4-2 antibody(ab46521)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NEDD4-2 antibody(ab46521) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-NEDD4-2 antibody(ab46521)Immunocytochemistry/ Immunofluorescence-NEDD4-2 antibody(ab46521) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab46521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.

Cellular localization

Cytoplasmic

Alternative names

  • E3 ubiquitin-protein ligase NEDD4-like protein antibody
  • NEDD4 2c antibody
  • NEDD4-2 antibody
  • NEDD4-2a antibody
  • NEDD4.2 antibody
  • NEDD4b antibody
  • NEDD4L antibody
  • NEDD4La antibody
  • NEDD4Lb antibody
  • NEDD4Lc antibody
  • NEDD4Ld antibody
  • NEDD4Le antibody
  • NEDD4Lf antibody
  • NEDD4Lg antibody
  • NEDD4Lh antibody
  • NEDL3 antibody
  • Neural precursor cell expressed, developmentally down regulated 4 like antibody
  • RSP5 antibody
see all

Anti-NEDD4-2 antibody images:

  Western blot - NEDD4-2 antibody (ab46521)

Western blot - NEDD4-2 antibody (ab46521)

All lanes : Anti-NEDD4-2 antibody (ab46521)

Lane 1 : Extract from non-transfected HEK293 cells
Lane 2 : Extract from HEK293 cells transfected with NEDD4-2


Predicted band size : 120 kDa
Observed band size : 120 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NEDD4-2 antibody(ab46521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NEDD4-2 antibody(ab46521)

ab46251 (1/1000) staining NEDD4-2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and membrane staining of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  Immunocytochemistry/ Immunofluorescence-NEDD4-2 antibody(ab46521)

Immunocytochemistry/ Immunofluorescence-NEDD4-2 antibody(ab46521)

ICC/IF image of ab46521 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46521, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NEDD4-2 antibody (ab46521)

This product has been referenced in:

  • Sidoryk-Wegrzynowicz Met al. Disruption of astrocytic glutamine turnover by manganese is mediated by the protein kinase C pathway. Glia 59:1732-43 (2011). WB; Human.Read more (PubMed: 21812036) »
  • Chandran Set al. Neural Precursor Cell-expressed Developmentally Down-regulated Protein 4-2 (Nedd4-2) Regulation by 14-3-3 Protein Binding at Canonical Serum and Glucocorticoid Kinase 1 (SGK1) Phosphorylation Sites. J Biol Chem 286:37830-40 (2011). WB; Mouse.Read more (PubMed: 21900244) »

See all 6 publications for this product

Publishing research using ab46521? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"