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Overview
Product name
Anti-NEDD4-2 antibody
See all NEDD4-2 products (6) ...
Description
Mouse monoclonal to NEDD4-2
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MATGLGEPVY GLSEDEGESR ILRVKVVSGI DLAKKDIFGA SDPYVKLSLY VADENRELAL VQTKTIKKTL NPKWNEEFYF RVNPSNHRLL FEVFDENRLT , corresponding to amino acids 1-100 of Human NEDD4-2
MATGLGEPVY GLSEDEGESR ILRVKVVSGI DLAKKDIFGA SDPYVKLSLY VADENRELAL VQTKTIKKTL NPKWNEEFYF RVNPSNHRLL FEVFDENRLT
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.
IP: Use at an assay dependent dilution. (PubMed: 19617352)
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Relevance
E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.
Cellular localization
Cytoplasmic
Alternative names
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- NEDD4 2 antibody
- NEDD4 2c antibody
see all
Database links
- Entrez Gene: 23327 Human
- Omim: 606384 Human
- SwissProt: Q96PU5 Human
- Unigene: 185677 Human
Anti-NEDD4-2 antibody images:
Western blot - NEDD4-2 antibody (ab58093)
Western blot against tagged recombinant protein immunogen using ab58093 NEDD4-2 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NEDD4-2 antibody(ab58093)
IHC image of ab58093 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58093, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-NEDD4-2 antibody (ab58093)
This product has been referenced in:
- Simonin A & Fuster D Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function. J Biol Chem 285:38293-303 (2010). WB; Human.Read more (PubMed: 20855896) »
- Caohuy Het al. Rescue of DeltaF508-CFTR by the SGK1/Nedd4-2 signaling pathway. J Biol Chem 284:25241-53 (2009). WB, IP; Human.Read more (PubMed: 19617352) »
See all 2 publications for this product
Publishing research using ab58093? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"