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Western blot - NEDD4-2 antibody (ab73386)
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Immunocytochemistry/ Immunofluorescence - NEDD4-2 antibody (ab73386)
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Product Name
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NEDD4-2 antibody
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See all NEDD4-2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NEDD4-2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NEDD4-2. (Peptide available as ab73385.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Chk
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
Spinal Cord (Mouse) Tissue; HepG2 Whole Cell; RAW 264.7 Whole Cell; EBS E14 TG2A Day 5 Whole cell
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NEDD4-2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NEDD4-2 antibody images, references, products related to ab73386 and other tools.
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NEDD4-2 antibody images:
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 Western blot - NEDD4-2 antibody (ab73386)
All lanes : NEDD4-2 antibody (ab73386) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 4 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 112 kDa
Observed band size : 112 kDa
Additional bands at : 44 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
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Immunocytochemistry/ Immunofluorescence - NEDD4-2 antibody (ab73386)
ICC/IF image of ab73386 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73386, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to NEDD4-2
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NEDD4-2 antibody - more information
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Customer reviews (feedback) regarding NEDD4-2 antibody |
Customer FAQs regarding NEDD4-2 antibody |
Protocols for NEDD4-2 antibody |
Price and availability of products related to NEDD4-2 antibody |
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NEDD4-2 antibody - related products:
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NEDD4-2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NEDD4-2 antibody |
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