Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-NEDD4-2 antibody
See all NEDD4-2 products (6) ...
Description
Rabbit polyclonal to NEDD4-2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chicken, Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NEDD4-2.
(Peptide available as ab73385.)
Positive control
This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue; HepG2 Whole Cell; RAW 264.7 Whole Cell; EBS E14 TG2A Day 5 Whole cell
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
Target
Relevance
E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.
Cellular localization
Cytoplasmic
Alternative names
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- NEDD4 2 antibody
- NEDD4 2c antibody
see all
Database links
- Entrez Gene: 374119 Chicken
- Entrez Gene: 23327 Human
- Entrez Gene: 83814 Mouse
- Omim: 606384 Human
- SwissProt: Q96PU5 Human
- SwissProt: Q8CFI0 Mouse
- Unigene: 185677 Human
- Unigene: 98668 Mouse
Anti-NEDD4-2 antibody images:
Western blot - NEDD4-2 antibody (ab73386)
All lanes : Anti-NEDD4-2 antibody (ab73386) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 4 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 112 kDa
Observed band size : 112 kDa
Additional bands at : 44 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
Immunocytochemistry/ Immunofluorescence - NEDD4-2 antibody (ab73386)
ICC/IF image of ab73386 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73386, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-NEDD4-2 antibody (ab73386)
This product has been referenced in:
- Eylenstein Aet al. Stimulation of Ca2+-channel Orai1/STIM1 by serum- and glucocorticoid-inducible kinase 1 (SGK1). FASEB J 25:2012-21 (2011). WB.Read more (PubMed: 21385992) »
See 1 publication for this product
Publishing research using ab73386? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"