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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Overview
Product name
Anti-NEDD4 antibody
See all NEDD4 products (5) ...
Description
Rabbit polyclonal to NEDD4
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Fusion protein: PVLLPTSSGL PPGWEEKQDD RGRSYYVDHN SKTTTWSKPT MQDDPRSKIP AHLRGKTPVD SNDLGPLP, corresponding to the WW2 domain, amino acids 395-462 of Rat NEDD4.
PVLLPTSSGL PPGWEEKQDD RGRSYYVDHN SKTTTWS KPT MQDDPRSKIP AHLRGKTPVD SNDLGPLP
Positive control
Mouse kidney, lung, and distal colon sections. Cytoplasmic extracts from mouse kidney cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 5mg/ml BSA, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/20000.Detects a band of approximately 115 kDa.(This antibody has been used to detected yeast(saccharomyces cerevisiae) Rsp-5 protein, a Nedd4 homologue. See Abreview.)
ICC/IF: Use at an assay dependent dilution. (See Abreview.)
Target
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains.
Domain
The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.
Post-translational
modifications
Auto-ubiquitinated.
Cellular localization
Cytoplasm. Cell membrane. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
Target information above from: UniProt accessionP46934
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 53 protein antibody
- E3 ubiquitin protein ligase Nedd4 antibody
- E3 ubiquitin-protein ligase NEDD4 antibody
see all
Database links
- Entrez Gene: 4734 Human
- Entrez Gene: 17999 Mouse
- Entrez Gene: 25489 Rat
- Omim: 602278 Human
- SwissProt: P46934 Human
- SwissProt: P46935 Mouse
- SwissProt: Q62940 Rat
- Unigene: 1565 Human
see all
References for Anti-NEDD4 antibody (ab14592)
This product has been referenced in:
- Tofaris GKet al. Ubiquitin ligase Nedd4 promotes alpha-synuclein degradation by the endosomal-lysosomal pathway. Proc Natl Acad Sci U S A 108:17004-9 (2011).Read more (PubMed: 21953697) »
- Mund T & Pelham HR Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2. Proc Natl Acad Sci U S A 107:11429-34 (2010). WB; Human.Read more (PubMed: 20534535) »
See all 7 publications for this product
Publishing research using ab14592? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"