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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Overview
Product name
Anti-NEDD4 antibody
See all NEDD4 products (5) ...
Description
Rabbit polyclonal to NEDD4
Tested applications
IHC-FoFr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Rabbit
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human NEDD4.
(Peptide available as ab30939.)
Positive control
HeLa Whole Cell Lysate (Human epithelial carcinoma cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: 1/1000
WB: Use a concentration of 1 µg/mlDetects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains.
Domain
The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.
Post-translational
modifications
Auto-ubiquitinated.
Cellular localization
Cytoplasm. Cell membrane. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
Target information above from: UniProt accessionP46934
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 53 protein antibody
- E3 ubiquitin protein ligase Nedd4 antibody
- E3 ubiquitin-protein ligase NEDD4 antibody
see all
Database links
- Entrez Gene: 4734 Human
- Entrez Gene: 17999 Mouse
- Entrez Gene: 25489 Rat
- Omim: 602278 Human
- SwissProt: P46934 Human
- SwissProt: P46935 Mouse
- SwissProt: Q62940 Rat
- Unigene: 1565 Human
see all
Anti-NEDD4 antibody images:
Western blot - NEDD4 antibody (ab27979)
Anti-NEDD4 antibody (ab27979) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 115 kDa
Observed band size : 115 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - NEDD4 antibody (ab27979)
ab27979 staining NEDD4 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. The antibody produced a diffuse and widespread staining in many brain areas. The image shows the staining obtained in the hippocampus part of brain.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet
Immunocytochemistry/ Immunofluorescence-NEDD4 antibody(ab27979)
ICC/IF image of ab27979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27979, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-NEDD4 antibody (ab27979)
This product has been referenced in:
- Zeng Fet al. Nedd4 mediates ErbB4 JM-a/CYT-1 ICD ubiquitination and degradation in MDCK II cells. FASEB J 23:1935-45 (2009).Read more (PubMed: 19193720) »
See 1 publication for this product
Publishing research using ab27979? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"