NEDD8 antibody [ND23] (ab50280)

Product Name 

NEDD8 antibody [ND23]

Product type 

Primary antibodies

Description 

Mouse monoclonal [ND23] to NEDD8

Immunogen 

Synthetic peptide: TDKVERIKERVEE conjugated to KLH, corresponding to amino acids 20-32 of Human NEDD8
BLAST 'TDKVERIKERVEE' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Cow, Rb, Zfsh
(Due to sequence homology)

Specificity 

ab50280 recognizes human Nedd8 and does not cross react with human SUMO1 or human ubiquitin

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.15 - 0.3 µg/ml. Predicted molecular weight: 9 kDa.
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Recombinant GST-Nedd8

Cellular localization 

Nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

Relevance 

Ubiquitin like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12 and Hub1. Proteins of the second class include Parkin, RAD23 and DSK2, are designated ubiquitin domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog NEDD8 are activated by the E1 ubiquitin activating enzyme UBA2, that forms isopeptide linkages between thio esters. NEDD8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3 ubiquitin ligase complex called the Skp1 /Cul1/Cdc53 F box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ND23

Isotype 

IgG2b

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified immunoglobulin

Form 

Liquid

Concentration 

2.600 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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NEDD8 antibody [ND23] images:

References for NEDD8 antibody [ND23] (ab50280)

  This product has been used in:

Gomes AV et al. Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues. Mol Cell Proteomics 8:302-15 (2009). PubMed: 18931337

If you publish research using ab50280 please let us know so that we can cite the reference on this datasheet.

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