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Read our guarantee »Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Anti-NEDD8 antibody [ND23]
See all NEDD8 products (10) ...
Mouse monoclonal [ND23] to NEDD8
ab50280 recognizes human Nedd8 and does not cross react with human SUMO1 or human ubiquitin
Reacts with
Human
Predicted to work with
Mouse, Rat, Rabbit, Cow, Zebrafish
Synthetic peptide: TDKVERIKERVEE conjugated to KLH, corresponding to amino acids 20-32 of Human NEDD8
TDKVERIKER VEE
Recombinant GST-Nedd8
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration information loading...
Purified immunoglobulin
Monoclonal
ND23
IgG2b
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Western blot - NEDD8 antibody [ND23] (ab50280)
(enlarge)
Our Abpromise guarantee covers the use of ab50280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.15 - 0.3 µg/ml. Predicted molecular weight: 9 kDa.
Array: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Belongs to the ubiquitin family.
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
Nucleus. Mainly nuclear.
Target information above from: UniProt accessionQ15843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - NEDD8 antibody [ND23] (ab50280)
![Western blot - NEDD8 antibody [ND23] (ab50280)](/ps/datasheet/Images/50/ab50280/ab50280wb.gif)
All lanes : Anti-NEDD8 antibody [ND23] (ab50280) at 0.12 µg/ml
Lane 1 : Nedd8-GST
Lane 2 : Ubiquitin-HIS
Lane 3 : Sumo1 (transfected 293T cell extracts)
Secondary
Goat anti-mouse Fab, Alkaline phosphatase conjugate
Predicted band size : 9 kDa
Observed band size : 9 kDa
Additional bands at : 8 kDa. We are unsure as to the identity of these extra bands.
Developed using BCIP substrate.
This product has been referenced in:
See 1 publication for this product
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![Western blot - NEDD8 antibody [ND23] (ab50280)](/ps/datasheet/Images/50/ab50280/ab50280wb.gif)
All lanes : Anti-NEDD8 antibody [ND23] (ab50280) at 0.12 µg/ml
Lane 1 : Nedd8-GST
Lane 2 : Ubiquitin-HIS
Lane 3 : Sumo1 (transfected 293T cell extracts)
Secondary
Goat anti-mouse Fab, Alkaline phosphatase conjugate
Predicted band size : 9 kDa
Observed band size : 9 kDa
Additional bands at : 8 kDa. We are unsure as to the identity of these extra bands.
Developed using BCIP substrate.
0
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