You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-NEDD8 antibody [ND23] (ab50280)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-NEDD8 antibody [ND23]
See all NEDD8 products (10) ...

Description

Mouse monoclonal [ND23] to NEDD8

Specificity

ab50280 recognizes human Nedd8 and does not cross react with human SUMO1 or human ubiquitin

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Rabbit, Cow, Zebrafish

Immunogen

Synthetic peptide: TDKVERIKERVEE conjugated to KLH, corresponding to amino acids 20-32 of Human NEDD8

TDKVERIKER VEE

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Recombinant GST-Nedd8

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purification notes

Purified immunoglobulin

Clonality

Monoclonal

Clone number

ND23

Isotype

IgG2b

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

  • Western blot - NEDD8 antibody [ND23] (ab50280)Western blot - NEDD8 antibody [ND23] (ab50280) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 0.15 - 0.3 µg/ml. Predicted molecular weight: 9 kDa.
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Tissue specificity

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Sequence similarities

Belongs to the ubiquitin family.

Post-translational
modifications

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

Cellular localization

Nucleus. Mainly nuclear.

Target information above from: UniProt accessionQ15843 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • MGC104393 antibody
  • MGC125896 antibody
  • MGC125897 antibody
  • NED8 antibody
  • NEDD 8 antibody
  • NEDD-8 antibody
  • Nedd8 antibody
  • NEDD8_HUMAN antibody
  • Neddylin antibody
  • Neural precursor cell expressed developmentally down regulated 8 antibody
  • Neural precursor cell expressed developmentally down regulated gene 8 antibody
  • Neural precursor cell expressed developmentally down-regulated protein 8 antibody
  • Rub1 antibody
  • Ubiquitin like protein Nedd 8 antibody
  • Ubiquitin like protein Nedd8 antibody
  • Ubiquitin-like protein Nedd8 antibody
see all

Database links

see all

Anti-NEDD8 antibody [ND23] images:

  Western blot - NEDD8 antibody [ND23] (ab50280)

Western blot - NEDD8 antibody [ND23] (ab50280)

All lanes : Anti-NEDD8 antibody [ND23] (ab50280) at 0.12 µg/ml

Lane 1 : Nedd8-GST
Lane 2 : Ubiquitin-HIS
Lane 3 : Sumo1 (transfected 293T cell extracts)

Secondary
Goat anti-mouse Fab, Alkaline phosphatase conjugate

Predicted band size : 9 kDa
Observed band size : 9 kDa
Additional bands at : 8 kDa. We are unsure as to the identity of these extra bands.

Developed using BCIP substrate.

References for Anti-NEDD8 antibody [ND23] (ab50280)

This product has been referenced in:

  • Gomes AVet al. Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues. Mol Cell Proteomics 8:302-15 (2009).Read more (PubMed: 18931337) »

See 1 publication for this product

Publishing research using ab50280? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"