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Anti-NEDD8 antibody (ab25988)

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Overview

Product name

Anti-NEDD8 antibody
See all NEDD8 products (10) ...

Description

Rabbit polyclonal to NEDD8

Tested applications

ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

A 14 amino acid synthetic peptide from the human NEDD8 conjugated to Dip.

Positive control

Human brain.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

  • ShowHide

    IHC-P

     IHC-P: 1/500 - 1/1000.Pe...Read more →

    IHC-P: 1/500 - 1/1000.Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

Function

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Tissue specificity

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Sequence similarities

Belongs to the ubiquitin family.

Post-translational
modifications

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

Cellular localization

Nucleus. Mainly nuclear.

Target information above from: UniProt accessionQ15843 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • MGC104393 antibody
  • MGC125896 antibody
  • MGC125897 antibody
  • NED8 antibody
  • NEDD 8 antibody
  • NEDD-8 antibody
  • Nedd8 antibody
  • NEDD8_HUMAN antibody
  • Neddylin antibody
  • Neural precursor cell expressed developmentally down regulated 8 antibody
  • Neural precursor cell expressed developmentally down regulated gene 8 antibody
  • Neural precursor cell expressed developmentally down-regulated protein 8 antibody
  • Rub1 antibody
  • Ubiquitin like protein Nedd 8 antibody
  • Ubiquitin like protein Nedd8 antibody
  • Ubiquitin-like protein Nedd8 antibody
see all

Database links

References for Anti-NEDD8 antibody (ab25988)

ab25988 has not yet been referenced specifically in any publications.

Publishing research using ab25988? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"