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Anti-NEDD8 antibody (ab38634) at 1/100 dilution + HeLa cell lysates
Predicted band size : 9 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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