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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-NEDD8 antibody
See all NEDD8 products (10) ...
Description
Rabbit polyclonal to NEDD8
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH-conjugated synthetic peptide selected within aa 1-100 of human NEDD8.
Positive control
HeLa cell lysates and human hepatocarcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 9 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Tissue specificity
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Sequence similarities
Belongs to the ubiquitin family.
Post-translational
modifications
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
Cellular localization
Nucleus. Mainly nuclear.
Target information above from: UniProt accessionQ15843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC104393 antibody
- MGC125896 antibody
- MGC125897 antibody
see all
Database links
- Entrez Gene: 4738 Human
- Omim: 603171 Human
- SwissProt: Q15843 Human
- Unigene: 531064 Human
- Unigene: 75512 Human
Anti-NEDD8 antibody images:
Western blot - NEDD8 antibody (ab38634)
Anti-NEDD8 antibody (ab38634) at 1/100 dilution + HeLa cell lysates
Predicted band size : 9 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NEDD8 antibody (ab38634)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
References for Anti-NEDD8 antibody (ab38634)
ab38634 has not yet been referenced specifically in any publications.
Publishing research using ab38634? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"