Overview

  • Product name
    Anti-NEDL2 antibody
  • Description
    Rabbit polyclonal to NEDL2
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human NEDL2.

  • Positive control
    • Extracts from HepG2 and LOVO cells. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A549

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/10000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 176 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.
    • Tissue specificity
      Predominantly expressed in adult brain, lung and heart.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 C2 domain.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 2 WW domains.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • C2 and WW domain-containing protein 2 antibody
      • E3 ubiquitin protein ligase HECW2 antibody
      • E3 ubiquitin-protein ligase HECW2 antibody
      • HECT antibody
      • HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 antibody
      • Hecw2 antibody
      • HECW2_HUMAN antibody
      • KIAA130 antibody
      • KIAA1301 antibody
      • NEDD4 like E3 ubiquitin protein ligase 2 antibody
      • NEDD4 Related E3 Ubiquitin Ligase antibody
      • NEDD4-like E3 ubiquitin-protein ligase 2 antibody
      see all

    Images

    • ICC/IF image of ab92711 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92711 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-NEDL2 antibody (ab92711) at 1/500 dilution

      Lane 1 : HepG2 cell extracts
      Lane 2 : LOVO cell extracts
      Lane 3 : LOVO cell extracts with immunizing peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 176 kDa

    References

    ab92711 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92711.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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