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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-NEIL1 antibody
See all NEIL1 products (3) ...
Description
Goat polyclonal to NEIL1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: DIPSLEPEGTSAS, corresponding to amino acids 378-390 of Human NEIL1.
DIPSLEPEGT SAS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NEIL1 antibody (ab14753)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
WB
Application notes
Tested in peptide ELISA: antibody detection limit dilution 1:16,000.
IHC-P: Use at a concentration of 5 µg/ml.
Western Blot: preliminary experiments give ~60kDa band in human liver and brain lysates at 1ug/ml. However predicted band size = 44kDa (NP_078884)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the FPG family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ96FI4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA (apurinic or apyrimidinic site) lyase Neil1 antibody
- DNA glycosylase/AP lyase Neil 1 antibody
- DNA glycosylase/AP lyase Neil1 antibody
see all
Database links
- Entrez Gene: 79661 Human
- Omim: 608844 Human
- SwissProt: Q96FI4 Human
- Unigene: 512732 Human
Anti-NEIL1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NEIL1 antibody (ab14753)
ab14753 at 5ug/ml staining NEIL1 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.
References for Anti-NEIL1 antibody (ab14753)
ab14753 has not yet been referenced specifically in any publications.
Publishing research using ab14753? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"