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IHC-P - NEK2 antibody (ab55550)
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Western blot - NEK2 antibody (ab55550)
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Product Name
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NEK2 antibody
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See all NEK2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to NEK2
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Immunogen
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Recombinant fragment, corresponding to amino acids 331-445 of Human NEK2
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Reacts with
(species key)
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Hu
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Tested applications
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently.
WB: Use at a concentration of 1 - 5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear. Localizes at the centromere. Isoform 1 has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end missing in isoform 2 which is predominantly cytoplasmic.
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Research areas
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Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Relevance
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NEK2 is closely related in its catalytic domain to the serine/threonine
protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.44 - 0.44 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NEK2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NEK2 antibody images, references, products related to ab55550 and other tools.
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NEK2 antibody images:
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 IHC-P - NEK2 antibody (ab55550)
NEK2 antibody (ab55550) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human testis.
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Western blot - NEK2 antibody (ab55550)
Western blot against tagged recombinant protein immunogen using ab55550 NEK2 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa
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Search PubMed (MEDLINE) for references to NEK2
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NEK2 antibody - more information
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Customer reviews (feedback) regarding NEK2 antibody |
Customer FAQs regarding NEK2 antibody |
Protocols for NEK2 antibody |
Price and availability of products related to NEK2 antibody |
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NEK2 antibody - related products:
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NEK2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NEK2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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