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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-NEK2 antibody
See all NEK2 products (6) ...
Description
Sheep polyclonal to NEK2
Specificity
This antibody recognises both splice variants of human NEK2 (NEK2A and NEK2B).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: NLERRGRQLGEPEK, corresponding to amino acids 282-295 of Human NEK2
NLERRGRQLG EPEK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - NEK2 antibody (ab79903)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Target
Function
Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
Tissue specificity
Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Developmental stage
Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
Post-translational
modifications
It is unsure whether Thr-170 or Ser-171 is phosphorylated.
Cellular localization
Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
Target information above from: UniProt accessionP51955
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Serine threonine protein kinase Nek2 antibody
- HSPK 21 antibody
- HsPK21 antibody
see all
Database links
- Entrez Gene: 4751 Human
- Omim: 604043 Human
- SwissProt: P51955 Human
- Unigene: 153704 Human
Anti-NEK2 antibody images:
Western blot - NEK2 antibody (ab79903)
Predicted band size : 49 kDa
Observed band size : 49 kDa
Western blot analysis demonstrating the specificity of ab79903. The left hand blot uses recombinant His(6)-NEK2A and the right hand blot uses recombinant His(6)-NEK2B. Ab79903 was used at 0.5ug/ml.
References for Anti-NEK2 antibody (ab79903)
ab79903 has not yet been referenced specifically in any publications.
Publishing research using ab79903? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"