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Western blot - NEK2 antibody (ab79903)
(enlarge)
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Product Name
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NEK2 antibody
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See all NEK2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Sheep polyclonal to NEK2
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Immunogen
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Synthetic peptide: NLERRGRQLGEPEK, corresponding to amino acids 282-295 of Human NEK2
BLAST 'NLERRGRQLGEPEK' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody recognises both splice variants of human NEK2 (NEK2A and NEK2B).
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear. Localizes at the centromere. Isoform 1 has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end missing in isoform 2 which is predominantly cytoplasmic.
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Research areas
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Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Relevance
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NEK2 is closely related in its catalytic domain to the serine/threonine
protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Sheep
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NEK2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NEK2 antibody images, references, products related to ab79903 and other tools.
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NEK2 antibody images:
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 Western blot - NEK2 antibody (ab79903)
Predicted band size : 49 kDa
Observed band size : 49 kDa
Western blot analysis demonstrating the specificity of ab79903. The left hand blot uses recombinant His(6)-NEK2A and the right hand blot uses recombinant His(6)-NEK2B. Ab79903 was used at 0.5ug/ml.
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Search PubMed (MEDLINE) for references to NEK2
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NEK2 antibody - more information
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Customer reviews (feedback) regarding NEK2 antibody |
Customer FAQs regarding NEK2 antibody |
Protocols for NEK2 antibody |
Price and availability of products related to NEK2 antibody |
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NEK2 antibody - related products:
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NEK2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NEK2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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