NEK2 protein (His tag) (ab42599)

Product Name 

NEK2 protein (His tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length protein: Human NEK2 fused to N terminal His tag.

Specificity 

100 U/mg. One unit is defined as the amount of enzyme that will
phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 0.3 mg/ml MBPsubstrate, and 0.2 ug/ml NEK2.

Tested applications 
(see key)

Inhib

Application notes 
(see key)

Recommended dilutions
Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

Relevance 

NEK2 is closely related in its catalytic domain to the serine/threonine
protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers

Storage buffer 

Preservative: None
Constituents: 40% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

70% SDS-PAGE

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Expressed in a Baculovirus infected Sf9 cell expression system.

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