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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - NELFe antibody (ab28038)
All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution
Lane 1 : NELFe-GST fusion protein
Lane 2 : HeLa cell nuclear extract
Secondary
donkey anti-sheep peroxidase linked at 1/20000 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)
HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI
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