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Products:Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Overview
Product name
Anti-NELFe antibody
See all NELFe products (2) ...
Description
Sheep polyclonal to NELFe
Tested applications
ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Fusion protein (Human)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/800
IP: Use at an assay dependent dilution.
WB: 1/1000Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
Target
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Tissue specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain.
Domain
The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18615
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
see all
Database links
- Entrez Gene: 7936 Human
- Omim: 154040 Human
- SwissProt: P18615 Human
- SwissProt: Q5JP74 Human
- SwissProt: Q5JP75 Human
- Unigene: 423935 Human
Anti-NELFe antibody images:
Western blot - NELFe antibody (ab28038)
All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution
Lane 1 : NELFe-GST fusion protein
Lane 2 : HeLa cell nuclear extract
Secondary
donkey anti-sheep peroxidase linked at 1/20000 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)
HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI
References for Anti-NELFe antibody (ab28038)
ab28038 has not yet been referenced specifically in any publications.
Publishing research using ab28038? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"