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Western blot - NELFe antibody (ab28038)
Western blot - NELFe antibody (ab28038)
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Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)
Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)
(enlarge)


Product Name 

NELFe antibody

 

See all NELFe antibodies (2)...

Product type 

Primary antibodies

Description 

Sheep polyclonal to NELFe

Immunogen 

Fusion protein (Human)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/800.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Elongation factors

 

Relevance 

Negative elongation factor E NELFe (also know as RD protein) is one of the five polypeptides that comprise the NELF complex. This complex is involved in the negative regulation of RNA polymerase II transcriptional elongation. It interacts with the DSIF complex to repress transcription. Its activity is reversed by positive transcription elongation factor B (PTEF-B), which is involved in phosphorylation of RNA pol II C-terminal domain.

 

Alternative names NELFe antibody (ab28038)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

7936    (Human)

GeneCard

    

GC06M032027    (Human)

Omim

    

154040    (Human)

SwissProt

    

P18615    (Human)
Q5JP74    (Human)
Q5JP75    (Human)

Unigene

    

423935    (Human)

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NELFe antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for NELFe antibody images, references, products related to ab28038 and other tools.

 

NELFe antibody images:

Product image  Western blot - NELFe antibody (ab28038)

Western blot - NELFe antibody (ab28038)

All lanes : NELFe antibody (ab28038) at 1/1000 dilution

Lane 1 : NELFe-GST fusion protein
Lane 2 : HeLa cell nuclear extract

Secondary
donkey anti-sheep peroxidase linked at 1/20000 dilution

Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)

Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)

HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI

 
 

Search PubMed (MEDLINE) for references to NELFe

 

NELFe antibody - more information

Customer reviews (feedback) regarding NELFe antibody

Customer FAQs regarding NELFe antibody

Protocols for NELFe antibody

Price and availability of products related to NELFe antibody

 

NELFe antibody - related products:

Products for Western blot

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Lysates

 

Compatible Secondaries for NELFe antibody

ab6743

  

FITC antibody

ab6745

  

Texas Red ® antibody

ab6746

  

Biotin antibody

ab6747

  

HRP antibody

ab6748

  

Alkaline Phosphatase antibody

ab7009

  

Phycoerythrin antibody

 

Other NELFe Antibodies

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NELFe antibody - other tools:

Contact Abcam with a Technical Enquiry about NELFe antibody

 

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