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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Anti-NELFe antibody
See all NELFe products (2) ...
Sheep polyclonal to NELFe
ICC/IF, IP, WBmore details
Reacts with
Human
Fusion protein (Human)
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Whole antiserum
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Our Abpromise guarantee covers the use of ab28038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/800
IP: Use at an assay dependent dilution.
WB: 1/1000Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Belongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain.
The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
Sumoylated.
Nucleus.
Target information above from: UniProt accessionP18615
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - NELFe antibody (ab28038)

All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution
Lane 1 : NELFe-GST fusion protein
Lane 2 : HeLa cell nuclear extract
Secondary
donkey anti-sheep peroxidase linked at 1/20000 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NELFe antibody (ab28038)

HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI
ab28038 has not yet been referenced specifically in any publications.
Publishing research using ab28038? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution
Lane 1 : NELFe-GST fusion protein
Lane 2 : HeLa cell nuclear extract
Secondary
donkey anti-sheep peroxidase linked at 1/20000 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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