Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Overview
Product name
Anti-NELFe antibody
See all NELFe products (2) ...
Description
Rabbit polyclonal to NELFe
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region between residue 330 and 380 of human NELFe (NP_002895.3)
Positive control
Human Breast Carcinoma, human Placenta
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
IHC-P
Application notes
IHC-P: 1/100 - 1/500.
Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Tissue specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain.
Domain
The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18615
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
see all
Database links
- Entrez Gene: 7936 Human
- Omim: 154040 Human
- SwissProt: P18615 Human
- SwissProt: Q5JP74 Human
- SwissProt: Q5JP75 Human
- Unigene: 423935 Human
Anti-NELFe antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NELFe antibody (ab84986)
ab84986 at 1/250 dilution staining NELFe in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NELFe antibody (ab84986)
ab84986 at 1/250 dilution staining NELFe in human placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
References for Anti-NELFe antibody (ab84986)
ab84986 has not yet been referenced specifically in any publications.
Publishing research using ab84986? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"