Overview

  • Product name
  • Description
    Rabbit polyclonal to NELFe
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 330 and 380 of human NELFe (NP_002895.3)

  • Positive control
    • Human Breast Carcinoma, human Placenta

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
    • Tissue specificity
      Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the RRM NELF-E family.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain
      The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Major histocompatibility complex gene RD antibody
      • Negative elongation factor E antibody
      • Negative elongation factor polypeptide E antibody
      • NELF e antibody
      • NELF-E antibody
      • Nelfe antibody
      • NELFE_HUMAN antibody
      • Nuclear protein antibody
      • RD antibody
      • RD protein antibody
      • RD RNA binding protein antibody
      • RDP antibody
      • RNA-binding protein RD antibody
      see all

    Images

    • ab84986 at 1/250 dilution staining NELFe in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab84986 at 1/250 dilution staining NELFe in human placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References

    ab84986 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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