Overview

  • Product nameNFAT1 peptide
  • DescriptionNFAT1 peptide

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab4905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • FormLiquid
  • Additional Notes


    This peptide may be used for neutralization and control experiments with the monoclonal antibody that reacts with this product and NFAT 1, catalog ab2722. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General info

  • Alternative names
    • AI607462
    • cytoplasmic 2
    • KIAA0611
    • NF ATp
    • NF-ATc2
    • NF-ATp
    • NFAC2_HUMAN
    • NFAT 1
    • NFAT pre existing subunit
    • NFAT pre-existing subunit
    • NFAT transcription complex, preexisting component
    • NFAT1
    • NFAT1-D
    • NFATc2
    • NFATc2
    • NFATp
    • NFATp
    • Nuclear factor of activated T cells cytoplasmic 2
    • Nuclear factor of activated T cells cytoplasmic 2
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2
    • Nuclear factor of activated T cells pre-existing component
    • Nuclear factor of activated T cells pre-existing component
    • Nuclear factor of activated T-cells
    • T cell transcription factor NFAT 1
    • T cell transcription factor NFAT 1
    • T-cell transcription factor NFAT1
    see all
  • FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
  • Tissue specificityExpressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
  • Sequence similaritiesContains 1 RHD (Rel-like) domain.
  • DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Target information above from: UniProt accession Q13469 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for NFAT1 peptide (ab4905)

ab4905 has not yet been referenced specifically in any publications.

Product Wall

The molecular weight of this peptide is ~2.4 kDa. Let me know if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"