You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameNFAT1 protein (Tagged-His Tag)See all NFAT1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 320-849 (530 amino acids) of Human NFAT1 with an N terminal His tag. Predicted mwt: 60 kDa; NCBI locus: NP_001129493.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 85 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesGLPRHIYPAVEFLGPCEQGERRNSAPESILLVPPTWPKPL VPAIPICSIPVTAS LPPLEWPLSSQSGSYELRIEVQPK PHHRAHYETEGSRGAVKAPTGGHPVVQLHG YMENKPLG LQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGN TKVLEI PLEPKNNMRATIDCAGILKLRNADIELRKGET DIGRKNTRVRLVFRVHIPESSG RIVSLQTASNPIECSQ RSAHELPMVERQDTDSCLVYGGQQMILTGQNFTSESKV VFTEKTTDGQQIWEMEATVDKDKSQPNMLFVEIPEYRNKH IRTPVKVNFYVING KRKRSQPQHFTYHPVPAIKTEPTD EYDPTLICSPTHGGLGSQPYYPQHPMVAES PSCLVATM APCQQFRTGLSSPDARYQQQNPAAVLYQRSKSLSPSLLGY QQPALM AAPLSLADAHRSVLVHAGSQGQSSALLHPSPT NQQASPVIHYSPTNQQLRCGSH QEFQHIMYCENFAPGT TRPGPPPVSQGQRLSPGSYPTVIQQQNA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91939 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AI607462cytoplasmic 2KIAA0611
        NF ATpNF-ATc2NF-ATpNFAC2_HUMANNFAT 1NFAT pre existing subunitNFAT pre-existing subunitNFAT transcription complex, preexisting componentNFAT1NFAT1-DNFATc2NFATpNFATpNuclear factor of activated T cells cytoplasmic 2Nuclear factor of activated T cells cytoplasmic 2Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2Nuclear factor of activated T cells pre-existing componentNuclear factor of activated T cells pre-existing componentNuclear factor of activated T-cellsT cell transcription factor NFAT 1T cell transcription factor NFAT 1T-cell transcription factor NFAT1
      see all
  • FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
  • Tissue specificityExpressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
  • Sequence similaritiesContains 1 RHD (Rel-like) domain.
  • DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications    In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

    • Target information above from: UniProt accession Q13469 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    NFAT1 protein (Tagged-His Tag) images

    • SDS-PAGE - NFAT1 protein (Tagged-His Tag) (ab91939)
      SDS-PAGE - NFAT1 protein (Tagged-His Tag) (ab91939)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for NFAT1 protein (Tagged-His Tag) (ab91939)

    ab91939 has not yet been referenced specifically in any publications.

    Publishing research using ab91939 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab91939 Submit a Question for ab91939

    There are currently no Abreviews or Questions for ab91939.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"