NFAT1 protein (Tagged-His Tag) (ab91939)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- AI607462cytoplasmic 2KIAA0611
- NF ATpNF-ATc2NF-ATpNFAC2_HUMANNFAT 1NFAT pre existing subunitNFAT pre-existing subunitNFAT transcription complex, preexisting componentNFAT1NFAT1-DNFATc2NFATpNFATpNuclear factor of activated T cells cytoplasmic 2Nuclear factor of activated T cells cytoplasmic 2Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2Nuclear factor of activated T cells pre-existing componentNuclear factor of activated T cells pre-existing componentNuclear factor of activated T-cellsT cell transcription factor NFAT 1T cell transcription factor NFAT 1T-cell transcription factor NFAT1
modificationsIn resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
NFAT1 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for NFAT1 protein (Tagged-His Tag) (ab91939)
ab91939 has not yet been referenced specifically in any publications.