Anti-NFATC2IP antibody (ab169140)
Key features and details
- Mouse polyclonal to NFATC2IP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFATC2IP antibody -
Description
Mouse polyclonal to NFATC2IP -
Host species
Mouse -
Specificity
In theory, the antibody could interact with isoform1 too since isoform2 is only a truncated version of isoform 1. Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey -
Immunogen
Recombinant full length protein corresponding to Human NFATC2IP aa 1 to the C-terminus.
Database link: Q8NCF5-2 -
Positive control
- ICC/IF: HeLa cells. WB: NFATC2IP transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa.
Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate). |
|
ICC/IF |
Use a concentration of 10 µg/ml. Antibody purification with Protein A will be needed prior to use.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate). |
ICC/IF
Use a concentration of 10 µg/ml. Antibody purification with Protein A will be needed prior to use. |
Target
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Function
Plays a role in the inducible expression of cytokine genes in T-cells, especially by increasing NFAT-driven IL-4 production. -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsMethylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production. -
Cellular localization
Nucleus. Cytoplasm. TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84901 Human
- Entrez Gene: 18020 Mouse
- Entrez Gene: 308983 Rat
- Omim: 614525 Human
- SwissProt: Q9GLZ9 Cynomolgus monkey
- SwissProt: Q8NCF5 Human
- SwissProt: O09130 Mouse
- SwissProt: Q6AYG7 Rat
see all -
Alternative names
- 45 kDa NF-AT-interacting protein antibody
- 45 kDa NFAT-interacting protein antibody
- cytoplasmic 2-interacting protein antibody
see all
Images
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Immunofluorescence analysis of permeabilized, paraformaldehyde-fixed HeLa cells, labeling NFATC2IP with ab169140 at 10 µg/ml.
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All lanes : Anti-NFATC2IP antibody (ab169140) at 1 µg/ml
Lane 1 : NFATC2IP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab169140 has been referenced in 1 publication.
- Jiang N et al. BUB1 drives the occurrence and development of bladder cancer by mediating the STAT3 signaling pathway. J Exp Clin Cancer Res 40:378 (2021). PubMed: 34852826