Anti-NFYA antibody (ab6558)

Overview

• Product nameAnti-NFYA antibodySee all NFYA primary antibodies ...
• Description
  Rabbit polyclonal to NFYA
• SpecificityNo reaction occurs with B subunit.
• Tested applicationsWB, IP, ELISA, EMSA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
• Concentration information loading...
• PurityIgG fraction
• Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
• Sequence similaritiesBelongs to the NFYA/HAP2 subunit family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P23511 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4800 Human
  • Entrez Gene: 18044 Mouse
  • Omim: 189903 Human
  • SwissProt: P23511 Human
  • SwissProt: P23708 Mouse
  • Unigene: 10441 Human
  • Unigene: 4929 Mouse

• Alternative names
  CAAT box DNA binding protein subunit A antibodyCAAT box DNA-binding protein subunit A antibodyCBF A antibody

  CBF B antibodyCBFA antibodyCBFB antibodyCCAAT binding transcription factor subunit B antibodyFLJ11236 antibodyHAP 2 antibodyHAP2 antibodyHAP2 CCAAT binding protein antibodyNF YA antibodyNF-YA antibodyNFY protein chain A antibodyNFYA antibodyNFYA_HUMAN antibodyNUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibodyNuclear transcription factor Y alpha antibodyNuclear transcription factor Y antibodyNuclear transcription factor Y subunit A antibodyNuclear transcription factor Y subunit alpha antibodySez10 antibodyTranscription factor NF Y A subunit antibody

  see all

Anti-NFYA antibody images

• 

  Western blot - NFYA antibody (ab6558)
  Anti-NFYA antibody (ab6558) at 1/500 dilution + CaPan1 whole cell lysate at 50 µg
  
  Secondary
  HRP-conjugated Donkey anti-Rabbit. at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 39 kDa
  Observed band size : 39 kDa
  Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 10 minutes

  This image is courtesy of an anonymous Abreview.

  See Abreview