Overview

  • Product nameAnti-NFYA antibodySee all NFYA primary antibodies ...
  • Description
    Rabbit polyclonal to NFYA
  • SpecificityNo reaction occurs with B subunit.
  • Tested applicationsWB, IP, ELISA, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution.
1-2 ul/assay.

Target

  • FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similaritiesBelongs to the NFYA/HAP2 subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit A antibody
    • CAAT box DNA-binding protein subunit A antibody
    • CBF A antibody
    • CBF B antibody
    • CBFA antibody
    • CBFB antibody
    • CCAAT binding transcription factor subunit B antibody
    • FLJ11236 antibody
    • HAP 2 antibody
    • HAP2 antibody
    • HAP2 CCAAT binding protein antibody
    • NF YA antibody
    • NF-YA antibody
    • NFY protein chain A antibody
    • NFYA antibody
    • NFYA_HUMAN antibody
    • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
    • Nuclear transcription factor Y alpha antibody
    • Nuclear transcription factor Y antibody
    • Nuclear transcription factor Y subunit A antibody
    • Nuclear transcription factor Y subunit alpha antibody
    • Sez10 antibody
    • Transcription factor NF Y A subunit antibody
    see all

Anti-NFYA antibody images

  • Anti-NFYA antibody (ab6558) at 1/500 dilution + CaPan1 whole cell lysate at 50 µg

    Secondary
    HRP-conjugated Donkey anti-Rabbit. at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

References for Anti-NFYA antibody (ab6558)

This product has been referenced in:
  • Hou Y  et al. DNA Demethylation and USF Regulate the Meiosis-Specific Expression of the Mouse Miwi. PLoS Genet 8:e1002716 (2012). EMSA ; Mouse . Read more (PubMed: 22661915) »
  • Grskovic M  et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). EMSA ; Human . Read more (PubMed: 17784790) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (CaPan1)
Loading amount 50 µg
Specification CaPan1
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"