Anti-NFYB antibody (ab6559)

Overview

• Product nameAnti-NFYB antibodySee all NFYB primary antibodies ...
• Description
  Rabbit polyclonal to NFYB
• SpecificityMinimal reaction was observed by WB against the 35 kDa A subunit of NF-Y
• Tested applicationsWB, IP, ELISA, EMSA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). The sequence is: QQI SGV QQI QFS

  Run BLAST with Run BLAST with
• Positive controlCHO-7 cells that were cultured in the presence of cholesterol.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
• Concentration information loading...
• PurityIgG fraction
• Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
• Sequence similaritiesBelongs to the NFYB/HAP3 subunit family.
• DomainCan be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P25208 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4801 Human
  • Entrez Gene: 18045 Mouse
  • Omim: 189904 Human
  • SwissProt: P25208 Human
  • SwissProt: P63139 Mouse
  • Unigene: 84928 Human
  • Unigene: 245998 Mouse

• Alternative names
  CAAT box DNA binding protein subunit B antibodyCAAT box DNA-binding protein subunit B antibodyCBF A antibody

  CBF B antibodyCCAAT binding transcription factor subunit A antibodyHAP 3 antibodyHAP3 antibodyNF Y protein chain B antibodyNF YB antibodyNF-Y protein chain B antibodyNF-YB antibodyNFYB antibodyNFYB_HUMAN antibodyNuclear transcription factor Y beta antibodyNuclear transcription factor Y subunit B antibodyNuclear transcription factor Y subunit beta antibodyTranscription factor NF Y B subunit antibody

  see all

Anti-NFYB antibody images

• 

  Electrophoretic Mobility Shift Assay - Anti-NFYB antibody (ab6559)Image from Grskovic M et al., PLoS Genet. 2007 Aug;3(8):e145. Fig 6.; doi:10.1371/journal.pgen.0030145; August 31, 2007, PLoS Genet 3(8): e145.
  EMSA was performed with a double-stranded, biotin-labeled oligonucleotide in the presence of murine ES cell nuclear extracts and increasing amounts of anti-NFYB antibody (ab6559; 2 µg or 6 µg) or unrelated antibody.

Protocols

• Recommended WB protocol for ab6559