• Product name
  • Description
    Rabbit polyclonal to NFYB
  • Specificity
    Minimal reaction was observed by WB against the 35 kDa A subunit of NF-Y
  • Tested applications
    Suitable for: WB, IP, ELISA, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). The sequence is: QQI SGV QQI QFS

  • Positive control
    • CHO-7 cells that were cultured in the presence of cholesterol.



Our Abpromise guarantee covers the use of ab6559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent dilution.
ELISA 1/4000.
EMSA Use 2µl for 104 cells.


  • Function
    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similarities
    Belongs to the NFYB/HAP3 subunit family.
  • Domain
    Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit B antibody
    • CAAT box DNA-binding protein subunit B antibody
    • CBF A antibody
    • CBF B antibody
    • CCAAT binding transcription factor subunit A antibody
    • HAP 3 antibody
    • HAP3 antibody
    • NF Y protein chain B antibody
    • NF YB antibody
    • NF-YB antibody
    • NFYB antibody
    • NFYB_HUMAN antibody
    • Nuclear transcription factor Y beta antibody
    • Nuclear transcription factor Y subunit B antibody
    • Nuclear transcription factor Y subunit beta antibody
    • Transcription factor NF Y B subunit antibody
    see all

Anti-NFYB antibody images

  • EMSA was performed with a double-stranded, biotin-labeled oligonucleotide in the presence of murine ES cell nuclear extracts and increasing amounts of anti-NFYB antibody (ab6559; 2 µg or 6 µg) or unrelated antibody.

References for Anti-NFYB antibody (ab6559)

This product has been referenced in:
  • Huang Y  et al. Phospho-?Np63a is a key regulator of the cisplatin-induced microRNAome in cancer cells. Cell Death Differ 18:1220-30 (2011). WB ; Human . Read more (PubMed: 21274007) »
  • Grskovic M  et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). EMSA ; Mouse . Read more (PubMed: 17784790) »

See all 2 Publications for this product

Product Wall

I'm sorry to hear you are having problems with ab6559. The protein NF-YB is know to function as a heterotrimeric transcription factor when bound to NF-YA and NF-YC. It is therefore possible that the large protein observed is made of NF-YB and other ...

Read More


Sign up