Overview

  • Product nameAnti-NFYB antibody
    See all NFYB primary antibodies
  • Description
    Rabbit polyclonal to NFYB
  • SpecificityMinimal reaction was observed by WB against the 35 kDa A subunit of NF-Y
  • Tested applicationsWB, IP, ELISA, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). The sequence is: QQI SGV QQI QFS

  • Positive control
    • CHO-7 cells that were cultured in the presence of cholesterol.

Properties

Applications

Our Abpromise guarantee covers the use of ab6559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent dilution.
ELISA 1/4000.
EMSA Use 2µl for 104 cells.

Target

  • FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similaritiesBelongs to the NFYB/HAP3 subunit family.
  • DomainCan be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAAT box DNA binding protein subunit B antibody
    • CAAT box DNA-binding protein subunit B antibody
    • CBF A antibody
    • CBF B antibody
    • CCAAT binding transcription factor subunit A antibody
    • HAP 3 antibody
    • HAP3 antibody
    • NF Y protein chain B antibody
    • NF YB antibody
    • NF-Y protein chain B antibody
    • NF-YB antibody
    • NFYB antibody
    • NFYB_HUMAN antibody
    • Nuclear transcription factor Y beta antibody
    • Nuclear transcription factor Y subunit B antibody
    • Nuclear transcription factor Y subunit beta antibody
    • Transcription factor NF Y B subunit antibody
    see all

Anti-NFYB antibody images

  • EMSA was performed with a double-stranded, biotin-labeled oligonucleotide in the presence of murine ES cell nuclear extracts and increasing amounts of anti-NFYB antibody (ab6559; 2 µg or 6 µg) or unrelated antibody.

References for Anti-NFYB antibody (ab6559)

This product has been referenced in:
  • Huang Y  et al. Phospho-?Np63a is a key regulator of the cisplatin-induced microRNAome in cancer cells. Cell Death Differ 18:1220-30 (2011). WB ; Human . Read more (PubMed: 21274007) »
  • Grskovic M  et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). EMSA ; Mouse . Read more (PubMed: 17784790) »

See all 2 Publications for this product

Product Wall

I'm sorry to hear you are having problems with ab6559. The protein NF-YB is know to function as a heterotrimeric transcription factor when bound to NF-YA and NF-YC. It is therefore possible that the large protein observed is made of NF-YB and other ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"