NFkB p100 / p52 antibody - ChIP Grade (ab7972)

Product Name 

NFkB p100 / p52 antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to NFkB p100 / p52 - ChIP Grade

Immunogen 

Synthetic peptide: VFLQLKRKRGGDVSDSKQFT, corresponding to C terminal amino acids 305-324 of Mouse NFkB p100 / p52(Peptide available as ab8007.)
BLAST 'VFLQLKRKRGGDVSDSKQFT' with or

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Reacts with NFkB p52 and p100

Tested applications 
(see key)

ChIP, ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 18850073).
IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: 1/200. Can be blocked with NF-kB p52 (C-term) peptide (mouse). Use 5% BSA 1hr to block PVDF membrane. Detects two bands of approximately 50kDa and 100 kDa (predicted molecular weight: 50 kDa).
ChIP: Use at an assay dependent dilution (from PubMed: 16713561).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat, Raji, Daudi cell lysates.

Cellular localization 

Cytoplasmic and Nuclear.

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway

Cell Biology >> Apoptosis >> Intracellular >> NFkB >> p52

Relevance 

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

Notes 

Members of the rel/NFkappaB family of transcription factors are involved in the regulation of cellular responses, such as growth, development, and the inflammatory response. They share a structural motif known as the rel homology region (RHR), the C-terminal one third of which mediates protein dimerization. Complexes of p50 (NF-kB1) or p52 (NF-kB2) are generated through the processing of p105 and p100 precursors, respectively. These are usually associated with members of the Rel family (p65, c-Rel, Rel B). The homo- and heterodimer formed through combinations of NF-kB/Rel proteins bind distinct kB sites to regulate the transcription of different genes.

References for NFkB p100 / p52 antibody - ChIP Grade (ab7972)

  This product has been used in: (two most recent references)

Svensson A & Tgerud S Galectin-1 expression in innervated and denervated skeletal muscle. Cell Mol Biol Lett 14:128-38 (2009). WB, ICC/IF; Human. PubMed: 18850073

Meir T et al. Molecular characteristics of liver metastases from uveal melanoma. Invest Ophthalmol Vis Sci 48:4890-6 (2007). PubMed: 17962435

See all 3 publication references for this product.


If you publish research using ab7972 please let us know so that we can cite the reference on this datasheet.

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