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Immunohistochemistry (Paraffin-embedded sections) - NFkB p100/p52 antibody (ab31409)
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Western blot - NFkB p100/p52 antibody (ab31409)
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Immunocytochemistry/ Immunofluorescence-NFkB p100 / p52 antibody(ab31409)
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Product Name
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NFkB p100 / p52 antibody
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See all NFkB p100 / p52 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NFkB p100 / p52
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Immunogen
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Synthetic non-phosphopeptide derived from Human NFkB p100/p52 around the phosphorylation site of Serine 869.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Antibody detects endogenous levels of NFkB p100/p52 protein around Serine 869.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts of Hela cells, MOLT cells and MDA-MB-435 cells. Breast carcinoma tissue.
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Cellular localization
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Cytoplasmic and Nuclear.
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Research areas
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Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Apoptosis >> Intracellular >> NFkB >> p52
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Relevance
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NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, Phosphate buffered saline (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NFkB p100 / p52 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NFkB p100 / p52 antibody images, references, products related to ab31409 and other tools.
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NFkB p100 / p52 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - NFkB p100/p52 antibody (ab31409)
ab31409 at 1/50, staining NFkB p100/p52 in paraffin embedded human breast carcinoma. Left image: Un-treated. Right image: Pre-incubated with synthesized peptide.
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Western blot - NFkB p100/p52 antibody (ab31409)
All lanes : NFkB p100 / p52 antibody (ab31409) at 1/500 dilution
Lane 1 : Extracts of Hela cells.
Lane 2 : Extracts of MOLT cells.
Lane 3 : Extracts of MDA-MB-435 cells treated with TNF alpha.
Predicted band size : 54 kDa
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Immunocytochemistry/ Immunofluorescence-NFkB p100 / p52 antibody(ab31409)
ICC/IF image of ab31409 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31409, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
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Search PubMed (MEDLINE) for references to NFkB p100 / p52
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NFkB p100 / p52 antibody - more information
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