NFkB p100 / p52 antibody (ab31432)

Product Name 

NFkB p100 / p52 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to NFkB p100 / p52

Immunogen 

Synthetic non phosphopeptide, 14 amino acids long, derived from Human NFkB p100 / p52 around the phosphorylation site of Serine 865.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody detects sites on p100 only. Sometimes there is a 52kDa band on WB; this is not p52. There is a cleavage site on the NFKB2 (Swiss-Prot No.: Q00653). The cleavage site is between 454 and 455 (cleavage when cotranslationally processed). The antibody can interact with the C-half sometimes. The MW. of C-half is near 52Kd.

Tested applications 
(see key)

ELISA, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects bands at 52 and 100kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: extract of ovary cancer cells.
IHC: and breast carcinoma tissue.

Cellular localization 

Cytoplasmic and Nuclear.

Research areas 

Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway

Cell Biology >> Apoptosis >> Intracellular >> NFkB >> p52

Relevance 

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, Phosphate buffered saline (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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